Immunocytochemical localization of gamma-glutamyltranspeptidase during fetal development of mouse kidney.
Immunocytochemical localization of gamma-glutamyltranspeptidase during fetal development of mouse kidney.
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DOI：
10.1177/36.2.2891746
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发表时间：
1988-02
期刊：
影响因子：
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通讯作者：
Karen Curto;W. Sweeney;E. Avner;N. Piesco;N. Curthoys
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文献类型：
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作者：
Karen Curto;W. Sweeney;E. Avner;N. Piesco;N. Curthoys
在发育完全的肾脏中，γ-谷氨酰转肽酶主要定位于近端小管的顶端质膜。使用灵敏的荧光测定法定量小鼠胎肾发生过程中这种活性的出现，并通过免疫细胞化学评估膜极性的发展。转肽酶在13天胎肾的比活性约为1 mU/mg蛋白质。在妊娠13 - 21天之间，总转肽酶活性增加了7500倍，而比活性增加了50倍。在妊娠13天，γ-谷氨酰转肽酶免疫反应性定位于发育中的肾泡的顶面和S形小管的近端段。有组织的细胞结构有紧密的管状连接，但缺乏明确的刷状缘膜。到妊娠15天，免疫染色的顶端表面的近端节段的发展是更加突出的，和轻微的反应性的基底外侧膜是明显的。到妊娠17天，肾脏被组织成离散的区域。γ-谷氨酰转肽酶活性的大幅增加与内皮层中近端小管的发育刷状缘膜的免疫染色增加相关。在后肾器官培养中观察到非常相似的转肽酶活性和免疫染色的出现模式，尽管有些延迟。近端小管囊肿形成的诱导对器官型肾发生过程中发生的转肽酶活性的增加没有影响。
In the fully developed kidney, gamma-glutamyltranspeptidase is localized predominantly to the apical plasma membrane of the proximal tubules. The appearance of this activity during murine fetal nephrogenesis was quantitated using a sensitive fluorometric assay, and development of membrane polarity was assessed by immunocytochemistry. Specific activity of the transpeptidase in 13-day fetal kidney was approximately 1 mU/mg protein. Between 13-21 days of gestation, total transpeptidase activity increased 7500-fold, whereas specific activity increased 50-fold. At 13 days of gestation, gamma-glutamyltranspeptidase immunoreactivity is localized to the apical surfaces of developing renal vesicles and the proximal segment of the S-shaped tubules. The organized cell structures have tight tubular junctions but lack a well-defined brush-border membrane. By 15 days of gestation, immunostaining of the apical surface of developing proximal segments is more prominent, and slight reactivity of the basolateral membrane is evident. By 17 days of gestation, the kidney is organized into discrete zones. The large increase in gamma-glutamyltranspeptidase activity correlates with the appearance of increased immunostaining of the developing brush-border membranes of the proximal tubules contained in the inner cortex. A very similar although somewhat delayed pattern of appearance of transpeptidase activity and immunostaining was observed in metanephric organ culture. Induction of proximal tubular cyst formation had no effect on the increase in transpeptidase activity that occurred during organotypic nephrogenesis.