Evaluation of commercial kava extracts and kavalactone standards for mutagenicity and toxicity using the mammalian cell gene mutation assay in L5178Y mouse lymphoma cells.
Evaluation of commercial kava extracts and kavalactone standards for mutagenicity and toxicity using the mammalian cell gene mutation assay in L5178Y mouse lymphoma cells.
复制标题
使用 L5178Y 小鼠淋巴瘤细胞中的哺乳动物细胞基因突变测定评估商业卡瓦提取物和卡瓦内酯标准品的致突变性和毒性。
DOI：
10.1016/j.fct.2007.07.013
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Dunkel,VirginiaC
中科院分区：
文献类型：
--
作者：
Whittaker,Paul;Clarke,JaneJ;San,RichardHC;Betz,JosephM;Seifried,HaroldE;deJager,LowriS;Dunkel,VirginiaC
Kava是胡椒家族的一员，南太平洋岛民种植了几个世纪，被用作社交和仪式饮料。传统上，卡瓦提取物是通过研磨或咀嚼根茎，并与水和椰奶混合制成的。Kava的活性成分是一组大约18种化合物，统称为Kavalactone或Kava吡喃。卡瓦因、二氢卡瓦因、甲硫菌素、二氢甲基卡瓦菌素、阳黄素和去甲氧基阳春素是六种主要的卡瓦拉酮类药物。众所周知，卡瓦饮料和其他制剂具有抗焦虑作用，可用于治疗焦虑症。含有卡瓦灌木根的膳食补充剂已被认为与几例人类肝脏毒性有关，其中包括几例在使用卡瓦补充剂后需要肝脏移植的病例。为了研究Kava的毒性和致突变性，用L5178Y小鼠淋巴瘤细胞进行了Kava和KavaPure两个市售样品，以及d-Kawain和dl-Kawain等6个纯Kavalactone的毒性和致突变性研究。在加入人肝S9活性的L5178Y小鼠淋巴瘤突变试验中，卡瓦样品和卡瓦拉酮都没有诱发突变反应。
Kava (Piper methysticum) is a member of the pepper family and has been cultivated by South Pacific islanders for centuries and used as a social and ceremonial drink. Traditionally, kava extracts are prepared by grinding or chewing the rhizome and mixing with water and coconut milk. The active constituents of kava are a group of approximately 18 compounds collectively referred to as kavalactones or kava pyrones. Kawain, dihydrokawain, methysticin, dihydromethysticin, yangonin, and desmethoxyyangonin are the six major kavalactones. Kava beverages and other preparations are known to be anxiolytic and are used for anxiety disorders. Dietary supplements containing the root of the kava shrub have been implicated in several cases of liver toxicity in humans, including several who required liver transplants after using kava supplements. In order to study the toxicity and mutagenicity, two commercial samples of kava, Kaviar and KavaPure, and the six pure kavalactones including both d-kawain and dl-kawain, were evaluated in L5178Y mouse lymphoma cells. Neither the kava samples nor the kavalactones induced a mutagenic response in the L5178Y mouse lymphoma mutation assay with the addition of human liver S9 activation.