X-ray structure of a voltage-dependent K+ channel
X-ray structure of a voltage-dependent K+ channel
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DOI：
10.1038/nature01580
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发表时间：
2003-05-01
期刊：
影响因子：
64.8
通讯作者：
MacKinnon, R
中科院分区：
文献类型：
--
作者：
Jiang, YX;Lee, A;MacKinnon, R
电压依赖性K+通道是电压依赖性阳离子（K+、Na+和Ca 2+）通道家族的成员，其响应于细胞膜电压的变化而打开并允许离子传导。这种形式的门控是神经和肌肉动作电位产生的基础。在这里，我们提出了KvAP的结构，电压依赖性K+通道从Aeropyrum pernix。我们已经确定了晶体结构的全长通道在3.2埃的分辨率，和隔离的电压传感器域在1.9埃，都在与单克隆Fab片段的复合物。该通道包含一个中央离子传导孔，周围环绕着电压传感器，形成了我们所说的“电压传感器桨”-通道外周上的疏水，阳离子，螺旋-转角-螺旋结构。柔性铰链表明，电压传感器桨移动响应膜电压的变化，携带正电荷穿过膜。
Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 Angstrom, and of the isolated voltage-sensor domain at 1.9 Angstrom, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.