Rare and fleeting: an example of interspecific recombination in animal mitochondrial DNA
Rare and fleeting: an example of interspecific recombination in animal mitochondrial DNA
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DOI：
10.1098/rsbl.2007.0290
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发表时间：
2007-10-22
期刊：
影响因子：
3.3
通讯作者：
Jordan, William C.
中科院分区：
文献类型：
--
作者：
Ciborowski, Kate L.;Consuegra, Sofia;Jordan, William C.
线粒体DNA（mtDNA）是一种特殊的DNA片段，在动物中很少发生。然而，检测mtDNA重组需要细胞通过突变、分子内重组或父系mtDNA的“泄漏”而变得异质。种间杂交增加了检测mtDNA重组体的可能性，这是由于更高水平的序列差异和潜在的更高水平的父系泄漏。在研究大西洋鲑鱼（萨尔莫salar）mtDNA的历史变异过程中，检测到一个具有包含大西洋鲑鱼和褐鳟鱼（萨尔莫trutta）序列的重组单倍型的个体。该个体不是F1杂种，但它确实具有不寻常的核基因型，这表明它是后代回交。在1948 - 2002年收集的717个个体中，没有发现来自同一种群或三个邻近大西洋鲑鱼种群的其他类似的重组单倍型。种间重组可能会增加物种内mtDNA的变异性，并可能对系统发育研究产生影响。
Recombination is thought to occur only rarely in animal mitochondrial DNA ( mtDNA). However, detection of mtDNA recombination requires that cells become heteroplasmic through mutation, intramolecular recombination or ' leakage' of paternal mtDNA. Interspecific hybridization increases the probability of detecting mtDNA recombinants due to higher levels of sequence divergence and potentially higher levels of paternal leakage. During a study of historical variation in Atlantic salmon ( Salmo salar) mtDNA, an individual with a recombinant haplotype containing sequence from both Atlantic salmon and brown trout ( Salmo trutta) was detected. The individual was not an F1 hybrid but it did have an unusual nuclear genotype which suggested that it was a later-generation backcross. No other similar recombinant haplotype was found from the same population or three neighbouring Atlantic salmon populations in 717 individuals collected during 1948 - 2002. Interspecific recombination may increase mtDNA variability within species and can have implications for phylogenetic studies.