Primary Structure and Functional Expression of the Apical Organic Cation Transporter from Kidney Epithelial LLC-PK1Cells*
Primary Structure and Functional Expression of the Apical Organic Cation Transporter from Kidney Epithelial LLC-PK1Cells*
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肾上皮 LLC-PK1 细胞顶端有机阳离子转运蛋白的一级结构和功能表达*
DOI：
10.1074/jbc.272.16.10408
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
E. Schömig
中科院分区：
文献类型：
--
作者：
D. Gründemann;J. Babin;F. Martel;N. Örding;A. Schmidt;E. Schömig
肾脏分泌的有机阳离子涉及至少两个不同的转运体，位于近端小管细胞的基底膜和顶膜上。尽管基侧转运蛋白最近已被克隆，但有关心尖型转运蛋白的序列信息尚未获得。从具有近端肾小管上皮细胞特性的猪细胞系LLC-PK1细胞中克隆到一种有机阳离子转运蛋白OCT2p。OCT2p在人胚胎肾293细胞中异源表达并鉴定。OCT2P介导的典型有机阳离子[14C]四乙基铵([14C]TEA)在293细胞中的摄取是饱和的。OCT2p对LLCP1和2 93细胞顶端[14C]TEA摄取抑制的Ki值呈高度正相关(r=0.995；p<；0.001；n=6)。虽然OCT2p在结构上与大鼠肾脏的底侧有机阳离子转运体OCT1r相关，但它们与皮质酮、去氢22、去甲基异丙肾上腺素在药理上有明显的区别。手头上的发现表明OCT2对应于有机阳离子转运蛋白的顶端类型。逆转录聚合酶链式反应表明，OCT1r的mRNA仅限于非神经组织，而OCT2r，即大鼠的OCT2p同源物，在肾脏和中枢神经区域都被发现，已知富含单胺递质多巴胺。
Renal secretion of organic cations involves at least two distinct transporters, located in the basolateral and apical membranes of proximal tubule cells. Whereas the basolateral transporter has recently been cloned, sequence information about the apical type was not yet available. An organic cation transporter, OCT2p, was cloned from LLC-PK1 cells, a porcine cell line with properties of proximal tubular epithelial cells. OCT2p was heterologously expressed and characterized in human embryonic kidney 293 cells. OCT2p-mediated uptake of the prototypical organic cation [14C]tetraethylammonium ([14C]TEA) into 293 cells was saturable. There was a highly significant correlation between the K i values for the inhibition of apical [14C]TEA uptake into LLC-PK1 cells and 293 cells transfected with OCT2p (r = 0.995;p < 0.001; n = 6). Although OCT2p is structurally related to OCT1r, the basolateral organic cation transporter from rat kidney, the transporters could be clearly discriminated pharmacologically with corticosterone, decynium22, andO-methylisoprenaline. The findings at hand suggest that OCT2 corresponds to the apical type of organic cation transporter. Reverse transcriptase-polymerase chain reaction indicates that mRNA of OCT1r is limited to non-neuronal tissue, whereas OCT2r, the OCT2p homologue from rat, was found in both the kidney and central nervous regions known to be rich in the monoamine transmitter dopamine.
兔肾刷状缘膜囊泡四乙铵/H交换器顺式和反式底物相互作用的机制。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wright,SH;Wunz,TM
通讯作者：
Wunz,TM
肾上皮细胞系运输有机阳离子（OK）。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yuan,G;Ott,RJ;Salgado,C;Giacomini,KM
通讯作者：
Giacomini,KM
在 LLC-PK1 细胞组织成上皮膜的过程中，碱性磷酸酶和 γ-谷氨酰转肽酶作为极化标记。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rabito,CA;Kreisberg,JI;Wight,D
通讯作者：
Wight,D