Localization of heat shock proteins in clinical Actinobacillus actinomycetemcomitans strains and their effects on epithelial cell proliferation
Localization of heat shock proteins in clinical Actinobacillus actinomycetemcomitans strains and their effects on epithelial cell proliferation
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DOI：
10.1111/j.1574-6968.2000.tb08900.x
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发表时间：
2000-01-15
影响因子：
2.1
通讯作者：
Uitto, VJ
中科院分区：
文献类型：
--
作者：
Paju, S;Goulhen, F;Uitto, VJ
伴放线放线杆菌是牙周炎的重要致病菌。在本研究中，我们定位的GroEL和DnaK样热休克蛋白（Hsp）的亚细胞组分12 A。activizor？？不同临床来源的vcetemconlitans菌株，并比较它们对牙周上皮细胞增殖和活力的影响。在所有菌株中，GroEL样蛋白被发现在膜，细胞质和周质，而DnaK样蛋白存在于细胞质和周质。Hsp的表达与A.伴放线菌菌株。表达GroEL样蛋白的细菌膜组分中度或强烈诱导上皮细胞增殖比弱表达该蛋白的菌株更强烈。上述结果提示，GroEL样Hsp可能在A.放线菌通过增加上皮细胞的增殖。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Actinobacillus actinomycetemcomitans is an important pathogen in periodontitis. In the present study we localized the GroEL- and DnaK-like heat shock proteins (Hsp) in subcellular fractions of 12 A. actiizor??vcetemconlitans strains of various clinical origin and compared their effects on periodontal epithelial cell proliferation and viability. In all strains, GroEL-like protein was found in the membrane, cytoplasm, and periplasm, whereas DnaK-like protein was present in the cytoplasm and periplasm. No correlation was observed between the Hsp expression and the serotype or origin of A. actinomycetemocomitans strains. The bacterial membrane fractions that expressed the GroEL-Iike protein moderately or strongly induced epithelial cell proliferation more strongly than strains that expressed the protein weakly. The results suggest that GroEL-like Hsp may play a role in the virulence of A. actinomycetemcomitans by increasing epithelial proliferation. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.