Methotrexate inhibits interleukin-6 production in patients with juvenile rheumatoid arthritis
Methotrexate inhibits interleukin-6 production in patients with juvenile rheumatoid arthritis
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DOI：
10.1007/s00296-002-0267-y
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发表时间：
2003-05-01
影响因子：
4
通讯作者：
Misra, R
中科院分区：
文献类型：
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作者：
Aggarwal, A;Misra, R
目的甲氨蝶呤（MTX）是治疗幼年类风湿性关节炎（JRA）应用最广泛的一类抗风湿药物。我们研究了它对JRA患者外周血细胞产生两种促炎细胞因子，白细胞介素-6 （IL-6）和肿瘤坏死因子α (TNFalpha)的影响。方法19例接受MTX（每周10 mg/m(2)）或安慰剂治疗的儿童，在有脂多糖（LPS）刺激和无脂多糖刺激的全血培养中，于0周和4周检测白细胞介素-6和TNFalpha水平。10名健康个体作为健康对照。结果JRA患者外周血细胞自发生成IL-6和TNFalpha高于正常对照组（P < 0.01）。然而，在健康对照和患者中，LPS刺激后IL-6和TNFalpha的产生是相似的。两组患者，即接受安慰剂治疗的患者和接受MTX治疗的患者，自发和诱导的IL-6和TNFalpha产生相似。在4周时，两组自发IL-6和TNFalpha产生的下降没有差异，但lps刺激的IL-6产生在mtx治疗组明显低于安慰剂组（P < 0.05）。结论甲氨蝶呤可减少活化细胞产生IL-6，这可能是其抗炎作用的原因。
Objectives Methotrexate (MTX) is one of the most widely used disease-modifying antirheumatoid drugs in the treatment of juvenile rheumatoid arthritis (JRA). We studied its effect on the production of two proinflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFalpha), by peripheral blood cells in patients with JRA. Methods Interleukin-6 and TNFalpha levels were measured at 0 and 4 weeks in whole blood cultures with and without lipopolysaccharide (LPS) stimulation in 19 children treated with MTX (10 mg/m(2) per week) or placebo. Ten healthy individuals were included as healthy controls. Results Spontaneous production of IL-6 and TNFalpha by peripheral blood cells of patients with JRA was higher than in healthy controls (P < 0.01). However, IL-6 and TNFalpha production after LPS stimulation was similar in healthy controls and patients. The two groups of patients, i.e., those treated with placebo and those treated with MTX, had similar spontaneous and induced IL-6 and TNFalpha production. At 4 weeks, the drop in spontaneous IL-6 and TNFalpha production was no different in the two groups, but LPS-stimulated IL-6 production was significantly lower in the MTX-treated group than the placebo group (P < 0.05). Conclusion Methotrexate reduces the production of IL-6 by activated cells, and this may be responsible for its anti-inflammatory property.