Measurement of polyunsaturated fatty acids of myocardial lipids by high performance liquid chromatography
Measurement of polyunsaturated fatty acids of myocardial lipids by high performance liquid chromatography
复制标题
高效液相色谱法测定心肌脂质多不饱和脂肪酸
DOI：
10.1007/bf02688569
复制
发表时间：
1987
期刊：
影响因子：
1.7
通讯作者：
D. Das
中科院分区：
文献类型：
--
作者：
G. Cordis;M. Renuka Prasad;H. Otani;R. Engelman;D. Das
摘要本报告描述了一种使用高效液相色谱法分离和分析20：4、20：5和22：6等多不饱和脂肪酸的改进方法。结果表明，这些脂肪酸与饱和酸很好地分离。由于不饱和脂肪酸比饱和酸更早地分解，并且该方法不需要使用薄层色谱法分离游离脂肪酸，这是气相色谱分析的必要步骤，因此与气相色谱法相比，多不饱和脂肪酸的回收率显著更高。此外，HPLC和气相色谱法得到了相同的结果磷脂酰丝氨酸的酰基链组成。本文还讨论了用高效液相色谱法测定游离脂肪酸和磷脂的酰基链组成的优点。
SummaryThis report describes a modified method for the separation and analysis of polyunsaturated fatty acids such as 20:4, 20:5, and 22:6, using HPLC. The results show that these fatty acids are well separated from the saturated acids. Since the unsaturated fatty acids elute earlier than saturated acids, and this method does not require the fractionation of free fatty acids using thin layer chromatography, a necessary step for the gas chromatographic analysis, the recoveries of polyunsaturated fatty acids were significantly higher as compared to those from gas chromatography. Furthermore, HPLC and gas chromatographic methods gave identical results for the acyl chain composition of phosphatidylserine. The advantages of using HPLC over gas chromatography in determining the acyl chain composition of free fatty acids and phospholipids are also discussed.