Monitoring thermal-induced changes in tumor blood flow and microvessels with laser speckle contrast imaging.
Monitoring thermal-induced changes in tumor blood flow and microvessels with laser speckle contrast imaging.
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DOI：
10.1364/ao.46.001911
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发表时间：
2007-04
期刊：
影响因子：
1.9
通讯作者：
Dan Zhu;W. Lu;Yang Weng;H. Cui;Qingming Luo
中科院分区：
文献类型：
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作者：
Dan Zhu;W. Lu;Yang Weng;H. Cui;Qingming Luo
采用激光散斑对比成像（LSCI）技术，在41℃~ 45℃的不同恒温条件下，监测正常小鼠和荷瘤小鼠在60 min的热诱导下肠系膜微血管血流和内径的变化，结果表明，血流和内径先增加后达到平稳，最后开始下降。温度越低，高原停留的时间越长。经t检验，同一组相对血流量和相对直径的平台值无显著差异。正常小鼠血流量和直径的相对增幅分别为1.26和1.41，荷瘤小鼠的相对增幅分别为1.08和1.13。在较高的处理温度或较长时间的热处理下，荷瘤小鼠的血流量和直径减少，这种变化比正常小鼠更为明显，说明肿瘤微血管对热的敏感性高于正常小鼠。微血管热收缩的发生往往快于血流的减少，直径的相对变化大于血流的相对变化。因此，我们可以得出结论，血管变形是改变微血管血流灌注的主要因素。
Laser speckle contrast imaging (LSCI) was used to monitor thermal-induced changes in the blood flow and the diameter of mesenteric microvessels of normal and tumor bearing mice under 60 min treatment at different constant temperatures between 41 degrees C and 45 degrees C. The results show that the blood flow and the diameter increase at the beginning and then reach a plateau and finally start to decrease. The lower the temperature, the longer the plateau stays. A t-test indicates that there is no significant difference in plateau values of relative blood flow and relative diameter for the same group. For normal mice, the relative increases in the blood flow and the diameter are 1.26 and 1.41, respectively, while for tumor-bearing mice they are 1.08 and 1.13, respectively. At higher treatment temperature or under longer heat treatment, there are decreases in the blood flow and the diameter, such changes in tumor-bearing mice are more obvious than those in normal mice, which means tumor microvessels are more sensitive to heat than normal. Moreover, thermal induced shrink of microvessel usually occurs sooner than the decrease in blood flow, and the relative change in diameter is larger than that in blood flow. Therefore we may conclude that deformation of vessel is a main factor for changing the blood perfusion of a microvessel.