A recombinant newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV
A recombinant newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV
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DOI：
10.1128/jvi.78.18.10054-10063.2004
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发表时间：
2004-09-01
影响因子：
5.4
通讯作者：
Samal, SK
中科院分区：
文献类型：
--
作者：
Huang, ZH;Elankumaran, S;Samal, SK
传染性法氏囊病病毒（IBDV）引起鸡的高度免疫抑制疾病。目前可获得的IBDV活疫苗可导致变异病毒的产生。我们已经开发出一种不会产生变异IBDV的替代疫苗。利用反向遗传学方法，从常用疫苗株LaSota中设计了重组新城疫病毒（NDV）载体，以表达变异体IBDV株GLS-5的宿主保护免疫原VP2。将编码IBDV VP2蛋白的基因插入全长NDV cDNA的最近3′位点，进行高水平表达。我们成功地恢复了重组病毒rLaSota/VP2。rLaSota/VP2基因稳定，在鸡胚中至少连续传代12次，并表达VP2蛋白。VP2蛋白未与重组病毒的病毒粒子结合。重组rLaSota/VP2在鸡胚和细胞培养中复制到与亲本NDV菌株LaSota相似的滴度。为了评估rLaSota/VP2的保护效果，在接种重组病毒后3周，用高毒力的NDV菌株Texas GB或IBDV变异株GLS-5攻毒2日龄的无特异性病原体鸡。接种rLaSota/VP2疫苗可产生针对NDV和IBDV的抗体应答，并对NDV和IBDV提供90%的保护。加强免疫诱导了更高水平的针对NDV和IBDV的抗体反应，并赋予了对这两种病毒的完全保护。这些结果表明，重组新城疫病毒可作为其他禽病原的疫苗载体。
Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota to express the host-protective immunogen VP2 of a variant IBDV strain GLS-5. The gene encoding the VP2 protein of the IBDV was inserted into the most 3'-proximal locus of a full-length NDV cDNA for high-level expression. We successfully recovered the recombinant virus, rLaSota/VP2. The rLaSota/VP2 was genetically stable, at least up to 12 serial passages in chicken embryos, and was shown to express the VP2 protein. The VP2 protein was not incorporated into the virions of recombinant virus. Recombinant rLaSota/VP2 replicated to a titer similar to that of parental NDV strain LaSota in chicken embryos and cell cultures. To assess protective efficacy of the rLaSota/VP2, 2-day-old specific-pathogen-free chickens were vaccinated with the recombinant virus and challenged with a highly virulent NDV strain Texas GB or IBDV variant strain GLS-5 at 3 weeks postvaccination. Vaccination with rLaSota/VP2 generated antibody responses against both NDV and IBDV and provided 90% protection against NDV and IBDV. Booster immunization induced higher levels of antibody responses against both NDV and IBDV and conferred complete protection against both viruses. These results indicate that the recombinant NDV can be used as a vaccine vector for other avian pathogens.