Effect of chitosan on the release of protein from thermosensitive poly (organophosphazene) hydrogels
Effect of chitosan on the release of protein from thermosensitive poly (organophosphazene) hydrogels
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DOI：
10.1016/j.ijpharm.2007.08.010
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发表时间：
2008-02-12
影响因子：
5.8
通讯作者：
Song, Soo-Chang
中科院分区：
文献类型：
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作者：
Kang, Gyung Don;Song, Soo-Chang
聚（有机磷腈）已被提议作为一种潜在的热敏水凝胶，用于开发可注射凝胶储库系统。在生物条件下，负载在水凝胶中的亲水性模型蛋白药物，包括牛血清白蛋白（BSA）、B型明胶（分子量20,000）（GB20）和异硫氰酸荧光素白蛋白（FITC - 白蛋白），释放时间为1 - 2周，呈现出初期的突释现象。然而，当蛋白质与壳聚糖形成复合物时，这种初期突释现象可以得到抑制，并且在这些条件下显示出延长的释放周期。BSA、GB20和FITC - 白蛋白在pH为7.4时都带负电荷，它们与带有正胺基的壳聚糖相互作用。通过测量ζ电位的变化和高效液相色谱法，证实了这些蛋白质/壳聚糖复合物的形成。我们确定抑制初期突释的蛋白质与壳聚糖的合适比例为1:5至1:10。从这些发现中，我们能够得出结论：通过形成蛋白质/壳聚糖复合物，可以控制释放速率和释放周期。（c）2007爱思唯尔有限公司。保留所有权利。
Poly(organophosphazenes) have been suggested as a potential thermosensitive hydrogel for use in the development of an injectable gel-depot system. Under biological conditions, hydrophilic model protein drugs, including bovine serum albumin (BSA), gelatin type B (MW 20,000) (GB20), and fluorescein isothiocyanate albumin (FITC-albumin) loaded in the hydrogels were released for 1-2 weeks, showing an initial burst release. However, this initial burst release could be suppressed when the proteins were couched in a complex with chitosan, and under these conditions evidenced a prolonged release period. BSA, GB20, and FITC-albumin, all of which are negatively charged at a pH of 7.4, interacted with chitosan harboring positive amine groups. The formation of these protein/chitosan complexes were confirmed via measurements of changes in zeta-potential and high-performance liquid chromatography. We determined the appropriate ratio of proteins to chitosan for suppression of the initial burst to be 1:5 to 1:10. From these findings, we were able to conclude that both the release rate and release period could be controlled via the formation of protein/chitosan complex. (c) 2007 Elsevier B.V. All rights reserved.