Overexpression of the eukaryotic translation initiation factor 4G (eIF4G-1) in squamous cell lung carcinoma
Overexpression of the eukaryotic translation initiation factor 4G (eIF4G-1) in squamous cell lung carcinoma
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DOI：
10.1002/ijc.10180
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发表时间：
2002-03-10
影响因子：
6.4
通讯作者：
Meese, E
中科院分区：
文献类型：
--
作者：
Bauer, C;Brass, N;Meese, E
eIF4G-1 属于翻译起始因子家族，被认为是翻译起始过程中 mRNA 募集的中心组织蛋白。先前发表的研究提供了一些证据，表明翻译因子的过度表达是癌发生过程中的普遍事件。我们通过蛋白质印迹法表征了 33 例肺鳞状细胞癌 (SCC) 中 eIF4G-1 蛋白的表达。与相应的正常肺组织相比，在 61% 的肿瘤中检测到 eIF4G-1 蛋白过度表达。此外，我们使用新生成的 eIF4G-1 特异性抗体，通过免疫组织化学分析了该蛋白在 138 个肺 SCC 中的表达，该抗体通过蛋白质印迹法测定。该抗 eIF4G-1 抗体适用于石蜡包埋组织的免疫组织化学分析。在肿瘤区域检测到强烈的细胞质染色，这与翻译因子 eIF4G-1 的细胞质定位一致。在 72% 的肺部鳞状细胞癌组织切片中，我们检测到与周围结缔组织相比 eIF4G-1 蛋白过度表达。通过两种方法分析的两个肿瘤均通过蛋白质印迹分析和免疫组织化学染色显示 eIF4G-1 过度表达。 eIF4G-1 的过度表达可能会导致翻译起始复合物 eIF4F 数量增加，进而可能激活与 eIF4E 相同的靶标 mRNA 的翻译。 (C) 2002 Wiley-Liss, Inc.
eIF4G-1 belongs to the family of translational initiation factors and is recognized as the central organizing protein in recruitment of mRNA during translational initiation. Previously published studies have provided some evidence that overexpression of translational factors is a general event in the process of carcinogenesis. We have characterized the expression of the eIF4G-1 protein in 33 squamous cell carcinoma (SCC) of the lung by Western blotting. Overexpression of the eIF4G-1 protein was detected in 61% of the tumors compared to the respective normal lung tissue. In addition, we analyzed the expression of this protein by immunohistochemistry in 138 SCC of the lung using a newly generated antibody that is specific for eIF4G-1 as determined by Western blotting. This anti-eIF4G-1 antibody was suitable for the immunohistochemistry of paraffin-embedded tissues. There is a strong cytoplasmic staining detected in the tumor areas that is consistent with the cytoplasmic localization of the translation factor eIF4G-1. In 72% of the examined tissue sections of SCCs of the lung, we detected an overexpression of the eIF4G-1 protein compared to the surrounding connective tissue. Two tumors that were analyzed by both methods showed an overexpression of eIF4G-1 both with Western blot analysis and immunohistochemical staining. Overexpression of eIF4G-1 may result in an increased amount of the translation initiation complex eIF4F, which in turn may activate the translation of the same target mRNAs as eIF4E. (C) 2002 Wiley-Liss, Inc.