Production of cyclodepsipeptides destruxin A and B from Metarhizium anisopliae
Production of cyclodepsipeptides destruxin A and B from Metarhizium anisopliae
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DOI：
10.1021/bp000087s
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发表时间：
2000-11-01
影响因子：
2.9
通讯作者：
Tzeng, YM
中科院分区：
文献类型：
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作者：
Liu, BL;Chen, JW;Tzeng, YM
麦芽糖和蛋白胨是金龟子绿僵菌生产destruxins的最佳碳源和氮源。基础培养基中添加0.1%（w/v）β-丙氨酸后，环缩酚肽DA和DB的产量分别为7.2和279 mg/L，是对照实验的2倍。应用响应面法 (RSM) 来优化摇瓶培养金龟子菌生产 DA 和 DB 时使用的麦芽糖、蛋白胨、β-丙氨酸和葡萄糖的组成。估计用于 DA 生产的最佳组成为麦芽糖 2.58%、蛋白胨 0.72%、β-丙氨酸 0.02% 和葡萄糖 0.55%。预测的 DA 产量为 18.5 mg/L。另一方面，DB生产的最佳组成为麦芽糖2.51%、蛋白胨0.75%、β-丙氨酸0.02%和葡萄糖0.43%。预计最大 DB 产量为 232 mg/L。这些通过在摇瓶中最大程度地产生德斯特鲁素的优化条件下进行的培养实验得到证实。此外，通过在无挡板搅拌罐发酵罐中采用响应面方法优化的 DB 生产培养基，获得了适度高水平的 DA (49 mg/L) 和 DB (268 mg/L) 产量。
Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae. With the addition of 0.1% (w/v) beta -alanine to the basal medium, the yields of cyclodepsipeptides DA and DB were 7.2 and 279 mg/ L, respectively, which was 2-fold higher than that of control experiment. Response surface methodology (RSM) was applied to optimize the compositions of maltose, peptone, beta -alanine, and glucose used in a shaker-flask cultivation of M. anisopliae for the production of DA and DB. Estimated optimal compositions for the DA production were maltose 2.58%, peptone 0.72%, beta -alanine 0.02%, and glucose 0.55%. The predicted DA yield was 18.5 mg/L. On the other hand, the optimal compositions for DB production were maltose 2.51%, peptone 0.75%, beta -alanine 0.02%, and glucose 0.43%. A maximum DB yield of 232 mg/L was predicted. These were confirmed by cultivation experiments conducted at the optimized conditions for maximum destruxins production in a shaker-flask. Furthermore, a modest high level of DA (49 mg/L) and DB (268 mg/L) yields were obtained by employing the response surface methodology optimized DB production medium in a no-baffle, stirred-tank fermenter.