Interaction of Bacillus thuringiensis Cry1 and Vip3A Proteins with Spodoptera frugiperda Midgut Binding Sites
Interaction of Bacillus thuringiensis Cry1 and Vip3A Proteins with Spodoptera frugiperda Midgut Binding Sites
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DOI：
10.1128/aem.02342-08
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发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Ferre, Juan
中科院分区：
文献类型：
--
作者：
Sena, Janete A. D.;Sara Hernandez-Rodriguez, Carmen;Ferre, Juan
测试Vip3Aa、Vip3Af、Cry1Ab和Cry1Fa的毒性和结合相互作用。Vip3A蛋白比Cry1蛋白毒性更强。结合试验显示Cry1和Vip3A蛋白具有独立的特异性结合位点。Cry1Ab和Cry1Fa竞争相同的结合位点，而Vip3Aa竞争Vip3Af的结合位点。
Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af.