Electroformation of giant phospholipid vesicles on a silicon substrate: Advantages of controllable surface properties
Electroformation of giant phospholipid vesicles on a silicon substrate: Advantages of controllable surface properties
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DOI：
10.1021/la703391q
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发表时间：
2008-03-18
期刊：
影响因子：
3.9
通讯作者：
Baigl, Damien
中科院分区：
文献类型：
--
作者：
Le Berre, Mael;Yamada, Ayako;Baigl, Damien
我们介绍了使用硅（Si）作为基板的巨型磷脂囊泡的电铸。通过利用硅表面性质的可调性，我们改变了电极上磷脂膜的组织，并研究了囊泡形成的后果。特别是，我们研究了硅表面化学和微观拓扑结构对磷脂膜的组织和最终囊泡的性质的影响。我们建立了化学均匀性，薄膜缺陷，和由此产生的囊泡大小分布之间的相关性。通过考虑磷脂膜，人工破碎的电极微结构，我们发现，囊泡的特征尺寸随着微结构尺寸的减小而减小。最后，我们提出了一种方法来控制囊泡的大小分布，通过使用硅衬底上的微图案化的二氧化硅层。
We introduce the use of silicon (Si) as a substrate for the electroformation of giant phospholipid vesicles. By taking advantage of the tunability of silicon surface properties, we varied the organization of the phospholipid film on the electrode and studied the consequences on vesicle formation. In particular, we investigated the effects of Si surface chemistry and microtopology on the organization of the phospholipid film and the properties of the final vesicles. We established correlations between chemical homogeneity, film defects, and resulting vesicle size distribution. By considering phospholipid films that are artificially fragmented by electrode microstructures, we showed that the characteristic size of vesicles decreases with a decrease in microstructure dimensions. We finally proposed a way to control the vesicle size distribution by using a micropatterned silicon dioxide layer on a Si substrate.