Monitoring dopamine release from single living vesicles with nanoelectrodes.
Monitoring dopamine release from single living vesicles with nanoelectrodes.
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DOI：
10.1021/ja050385r
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发表时间：
2005-06
影响因子：
15
通讯作者：
Wen-Zhan Wu;Weihua Huang;Wei Wang;Zongli Wang;Jie-ke Cheng;Tao Xu;Rong-Ying Zhang;Yu Chen
中科院分区：
文献类型：
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作者：
Wen-Zhan Wu;Weihua Huang;Wei Wang;Zongli Wang;Jie-ke Cheng;Tao Xu;Rong-Ying Zhang;Yu Chen
碳纤维纳米电极（尖端直径=约1.5 mm）100 nm）已首次用于监测实时多巴胺释放从单个大鼠嗜铬细胞瘤（PC12）细胞的单个活囊泡。实验表明，细胞表面存在活性和非活性释放位点，即使在同一活性释放区，其空间分布也有差异。首次证明了多个囊泡可以在细胞表面的同一位点顺序释放多巴胺，这可能在PC12细胞释放多巴胺中起主要作用。
Carbon fiber nanoelectrodes (tip diameter = ca. 100 nm) have been first used to monitor real-time dopamine release from single living vesicles of single rat pheochromocytoma (PC12) cells. The experiments show that active and inactive release sites exist on the surface of cells, and the spatial distributions have been differentiated even in the same active release zone. It is first demonstrated that multiple vesicles can sequentially release dopamine at the same site of the cell surface, which possibly plays the main role in the dopamine release from PC12 cells.