Genetic variation in Tanis was associated with elevating plasma triglyceride level in Chinese nondiabetic subjects.
Genetic variation in Tanis was associated with elevating plasma triglyceride level in Chinese nondiabetic subjects.
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DOI：
10.1186/1476-511x-12-97
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发表时间：
2013-07-05
影响因子：
4.5
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Gao Y;Xie X;Ma YT;Yang YN;Li XM;Fu ZY;Zheng YY;Ma X;Chen BD;Liu F;Huang Y
Tanis 基因多态性与人类甘油三酯浓度的关联尚未得到彻底检查。我们的目的是研究甘油三酯浓度与 Tanis 遗传多态性之间的关系。从参加心血管风险调查（CRS）研究的受试者中选出的所有参与者（n=1497）根据种族分为两组（汉族：n=1059；维吾尔族：n=438）。按照制造商的建议，使用 Applied Biosystems 的 TaqMan® 检测对 Tanis 基因的四个标记 SNP（rs12910524、rs1384565、rs2101171、rs4965814）进行基因分型，并在 ABI 7900HT 快速实时 PCR 系统中进行分析。通过显性模型（P<0.001）、隐性模型（P<0.001）和加性模型（P<0.001）分析，无论是在汉族还是在维吾尔族中，我们发现SNP rs12910524与甘油三酯水平存在相关性，并且在调整性别、年龄、饮酒量、吸烟、BMI和血浆葡萄糖（GLU）水平后，差异仍然显着（所有P < 0.001）。然而，在多变量调整前后，rs1384565、rs2101171和rs4965814并未观察到这种关系（均P > 0.05）。此外，汉族（P=0.001）和维吾尔族（P=2.60×10-4）中rs12910524与TG上的GLU之间存在显着的交互作用。我们的结果表明，Tanis 基因中的 rs12910524 与中国非糖尿病受试者的甘油三酯浓度相关。
The association of genetic polymorphisms of Tanis with triglyceride concentration in human has not been thoroughly examined. We aimed to investigate the relationship between triglyceride concentrations and Tanis genetic polymorphisms. All participants (n=1497) selected from subjects participating in the Cardiovascular Risk Survey (CRS) study were divided into two groups according to ethnicity (Han: n=1059; Uygur: n= 438). Four tagging SNPs (rs12910524, rs1384565, rs2101171, rs4965814) of Tanis gene were genotyped using TaqMan® assays from Applied Biosystems following the manufacturer’s suggestions and analyzed in an ABI 7900HT Fast Real-Time PCR System. We found that the SNP rs12910524 was associated with triglyceride levels by analyses of a dominant model (P<0.001), recessive model (P <0.001) and additive model (P < 0.001) not only in Han ethnic but also in Uygur ethnic group, and the difference remained significant after the adjustment of sex, age, alcohol intake, smoking, BMI and plasma glucose (GLU) level (All P < 0.001). However, this relationship was not observed in rs1384565, rs2101171, and rs4965814 before and after multivariate adjustment (All P > 0.05). Furthermore, there were significant interactions between rs12910524 and GLU on TG both in Han (P=0.001) and Uygur population (P=2.60×10-4). Our results indicated that the rs12910524 in the Tanis gene was associated with triglyceride concentrations in subjects without diabetes in China.