Effects of Treatment with Sodium Dodecyl Sulfate on the Ultrastructure of Escherichia coli
Effects of Treatment with Sodium Dodecyl Sulfate on the Ultrastructure of Escherichia coli
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DOI：
10.1128/jb.111.3.801-813.1972
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发表时间：
1972-09
影响因子：
3.2
通讯作者：
C. Woldringh;W. Iterson
中科院分区：
文献类型：
--
作者：
C. Woldringh;W. Iterson
用电子显微镜研究了十二烷基硫酸钠(十二烷基硫酸钠)溶解大肠杆菌质膜对核质和细胞质组织的影响。在时程实验中观察到的变化与吸光度的变化和细胞内大分子的释放有关。当细胞发生质壁分解时，在所用的条件下，十二烷基硫酸钠的第一个可见效应是质壁分解空间的塌陷。随之而来的是核材料的位移，然后看起来与重新部署的质膜有广泛的接触。核物质向细胞边界的这种初始移位可能表明核质和质膜之间的联系。当质膜进一步溶解时，核物质从细胞边缘后退，收缩成轴向细丝。同时，细胞质分裂成对Pronase敏感的无定形成分和对核糖核酸酶敏感的不透明电子颗粒成分。后者代表围绕核质的核糖体结构的一个连续区域，当细胞在十二烷基硫酸钠治疗前被利福霉素抑制时，这种组织不会出现。在长时间的SDS相互作用中，约65%的细胞蛋白质、25%的核糖核酸和40%的脱氧核糖核酸从细胞中释放出来，伴随着无定形细胞质部分的消失、核糖体聚集体的膨胀和细胞周围核物质的重排。这些观察结果支持了所有核糖体结构都与核质有直接关系的观点。
An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macromolecules from the cells. As the cells became plasmolyzed, under the conditions used, the first visible effect of SDS was a collapse of the plasmolysis spaces. This was accompanied by a displacement of the nuclear material which then appeared in broad contact with the redeployed plasma membrane. This initial displacement of nuclear material to the cell border may indicate an association between the nucleoplasm and the plasma membrane. Upon further dissolution of the plasma membrane, the nuclear material receded from the cell margin and contracted into an axial filament. Meanwhile, the cytoplasm dissociated into an amorphous, Pronase-sensitive component and an electron-opaque, granular one sensitive to ribonuclease. The latter represented one continuous area of ribosomal structures surrounding the nucleoplasm, an organization which did not occur when the cells were inhibited with rifamycin before SDS treatment. During prolonged SDS interaction, approximately 65% of the cellular protein, 25% of the ribonucleic acid and 40% of the deoxyribonucleic acid were released from the cells concomitant with the disappearance of the amorphous cytoplasmic part, expansion of the ribosomal aggregate, and rearrangement of the nuclear material at the cell periphery. The observations support the contention that all ribosomal structures bear a direct relationship with the nucleoplasm.