Impact of Cultivated Hosts on the Recombination of Cucumber Mosaic Virus
Impact of Cultivated Hosts on the Recombination of Cucumber Mosaic Virus
复制标题
DOI：
10.1128/jvi.01770-18
复制
发表时间：
2019-02
影响因子：
5.4
通讯作者：
R. S. Ouedraogo;J. Pita;I. Somda;O. Traoré;M. Roossinck
中科院分区：
文献类型：
--
作者：
R. S. Ouedraogo;J. Pita;I. Somda;O. Traoré;M. Roossinck
趋化是病毒多样化和适应不同宿主的重要机制。了解宿主在进化机制中的作用对于病毒疾病管理和控制新菌株的出现非常重要。这项研究表明了栽培宿主在CMV进化中发挥的影响。此外，我们的结果和以前的研究表明，一些特定的宿主可能是新病毒株出现的理想环境。黄瓜花叶病毒（CMV）是已知最成功的病毒之一，感染了1,200多种植物。与其他单链RNA病毒一样，CMV由于易于出错的复制和短的世代时间而具有很高的群体多样性潜力。寄生也是病毒适应新宿主的一种机制。已经通过使用单细胞酵母系统鉴定了影响RNA病毒重组的宿主基因。要确定的影响，天然植物宿主病毒重组，我们使用了高重组频率株的CMV，LS-CMV，它属于亚组II，在三个不同的栽培主机：辣椒annuum CV。马伦戈（胡椒）、烟草（Nicotiana tabacum cv.）Xanthi nc（烟草）和西葫芦Cucurbita pepo cv.黑美人（西葫芦）。通过使用携带通过引入沉默突变产生的限制性酶位点的RNA 3报告基因计算重组频率。结果表明，LS-CMV的重组频率与感染的寄主有关。辣椒中的重组事件是烟草中的1.8倍，是西葫芦中的5倍。此外，我们观察到在接种辣椒植物中产生缺陷RNA，但在烟草或西葫芦中没有。这些结果表明，宿主参与了分子内和分子间的重组事件，辣椒等宿主可以促进病毒更快的进化。此外，我们报告的第一次生产缺陷RNA的CMV亚组II隔离。重要性减弱是病毒多样化和适应不同宿主的重要机制。了解宿主在进化机制中的作用对于病毒疾病管理和控制新菌株的出现非常重要。这项研究表明了栽培宿主在CMV进化中发挥的影响。此外，我们的结果和以前的研究表明，一些特定的宿主可能是新病毒株出现的理想环境。
Recombination is an important mechanism used by viruses for their diversification and to adapt to diverse hosts. Understanding the host role in the mechanisms of evolution is important for virus disease management and controlling the emergence of new strains. This study shows the impact that cultivated hosts are playing in the evolution of CMV. Furthermore, our results and previous studies show how some specific hosts could be an ideal environment for the emergence of new viral strains. ABSTRACT Cucumber mosaic virus (CMV) is one of the most successful viruses known, infecting over 1,200 species of plants. Like other single-stranded RNA viruses, CMV is known to have a high potential for population diversity due to error-prone replication and short generation times. Recombination is also a mechanism that allows viruses to adapt to new hosts. Host genes have been identified that impact the recombination of RNA viruses by using single-cell yeast systems. To determine the impact that the natural plant host has on virus recombination, we used a high-recombination-frequency strain of CMV, LS-CMV, which belongs to subgroup II, in three different cultivated hosts: Capsicum annuum cv. Marengo (pepper), Nicotiana tabacum cv. Xanthi nc (tobacco), and Cucurbita pepo cv. Black Beauty (zucchini). The recombination frequency was calculated by using an RNA 3 reporter carrying restriction enzyme sites created by introducing silent mutations. Our results show that the recombination frequency of LS-CMV is correlated with the infected host. The recombination events in pepper were 1.8-fold higher than those in tobacco and 5-fold higher than those in zucchini. Furthermore, we observed the generation of defective RNAs in inoculated pepper plants, but not in tobacco or zucchini. These results indicate that the host is involved in both intra- and intermolecular recombination events and that hosts like pepper could foster more rapid evolution of the virus. In addition, we report for the first time the production of defective RNAs in a CMV subgroup II isolate. IMPORTANCE Recombination is an important mechanism used by viruses for their diversification and to adapt to diverse hosts. Understanding the host role in the mechanisms of evolution is important for virus disease management and controlling the emergence of new strains. This study shows the impact that cultivated hosts are playing in the evolution of CMV. Furthermore, our results and previous studies show how some specific hosts could be an ideal environment for the emergence of new viral strains.