Synaptic and extrasynaptic distribution of two distinct populations of nicotinic acetylcholine receptor clusters in the frog cardiac ganglion.
Synaptic and extrasynaptic distribution of two distinct populations of nicotinic acetylcholine receptor clusters in the frog cardiac ganglion.
复制标题
青蛙心脏神经节中两个不同烟碱乙酰胆碱受体簇群的突触和突触外分布。
DOI：
10.1007/bf02284786
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Sargent,PB
中科院分区：
文献类型：
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作者：
WilsonHorch,HL;Sargent,PB
我们研究了神经元烟碱乙酰胆碱受体簇的分布在青蛙心脏的自主神经元，使用免疫荧光技术和激光扫描共聚焦显微镜的突触部位。乙酰胆碱受体簇使用大鼠抗电泳乙酰胆碱受体单克隆抗体22号和花青3.18标记的山羊抗大鼠二抗进行可视化。用小鼠抗突触囊泡蛋白SV 2、单克隆抗体10 h和花青5.18标记的山羊抗小鼠二抗标记突触终扣。神经元表面上的乙酰胆碱受体簇存在于两个群体中，其大小、染色强度和表面分布不同。更突出的群体由每个细胞数量为30±15个的大的、明亮染色的簇组成，而第二类较小且染色不那么明亮，每个细胞数量超过100个。大型集群往往被组织成2-6个成员的组。这种排列是由于80%的大簇共定位在突触终扣处，并且单个终扣可以有几个相关的簇。剩下的20%的大/亮乙酰胆碱受体簇是突触外的，但它们也是聚集的，并且靠近突触终扣。小的/暗淡的乙酰胆碱受体簇随机分布在细胞表面。大/明亮的突触乙酰胆碱受体簇可能是快速兴奋性突触传递的基础。小/暗簇和大/亮突触外簇可能代表大/亮突触簇代谢的中间体。
We examined the distribution of neuronal nicotinic acetylcholine receptor clusters in relation to synaptic sites on autonomic neurons in the frog heart using immunofluorescence techniques and laser scanning confocal microscopy. Acetylcholine receptor clusters were visualized using the rat anti-Electrophorusacetylcholine receptor monoclonal antibody no. 22 and cyanine 3.18-labelled goat anti-rat secondary antibody. Synaptic boutons were labelled with the mouse anti-synaptic vesicle protein SV2, monoclonal antibody no. 10h and cyanine 5.18-labelled goat anti-mouse secondary antibody. Acetylcholine receptor clusters on the neuronal surface exist in two populations that vary in size, staining intensity, and surface distribution. The more prominent population consists of large, brightly stained clusters numbering 30±15 per cell, while the second class is smaller and less brightly stained and numbers over 100 per cell. The large clusters tend to be organized into groups of 2–6 members. This arrangement results from the fact that 80% of the large clusters colocalize at synaptic boutons and that single boutons can have several associated clusters. The remaining 20% of large/bright acetylcholine receptor clusters are extrasynaptic, but they, too, are clustered and are found in close proximity to synaptic boutons. The small/dim acetylcholine receptor clusters are randomly distributed over the cell surface. The large/bright synaptic acetylcholine receptor clusters presumably underlie fast excitatory synaptic transmission. The small/dim clusters and the large/bright extrasynaptic clusters may represent intermediates in the metabolism of large/bright synaptic clusters.