An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease
An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease
复制标题
OsPRMT5-OsAGO2/miR1875-OsHXK1 模块调节水稻对稻瘟病的免疫力
DOI：
10.1111/jipb.13430
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发表时间：
2023-01-19
影响因子：
11.4
通讯作者：
Zhao，Hongwei
中科院分区：
文献类型：
--
作者：
Sheng，Cong;Li，Xuan;Zhao，Hongwei
水稻ARGONAUTE2(OsAGO2)是水稻RNA诱导沉默复合体(RISC)的核心成分，受稻瘟病菌(Magnaporthe Oryzae)侵染抑制。OsAGO2介导的基因沉默是否以及如何在水稻抗稻瘟病中发挥作用，以及哪些sRNAs参与了这一过程尚不清楚。我们的结果表明，OsAGO2是控制水稻对稻瘟病的防御反应的关键免疫因子。OsAGO2与24nt的miR1875结合，并与HEXOKINASE 1(OsHXK1)的启动子区域结合，导致DNA甲基化，导致基因沉默。我们的多重基因证据表明，没有M。水稻感染后，OsAGO2/miR1875 RISC促进OsHXK1启动子DNA甲基化和OsHXK1沉默；OsHXK1是稻瘟病抗性的正调控因子，OsHXK1-OErice对稻瘟病的抗性增强，Cas9-Oshxk1对稻瘟病的抗性降低。稻瘟病。OsHXK1可能通过其糖感受器活性在日本晴和OsHXK1-OE中通过葡萄糖诱导防御相关基因表达和ROS积累发挥作用，但在Cas9-Oshxk1中不起作用。OsAGO2本身受到OsPRMT5的微妙调控，OsPRMT5是一种感觉M。通过OsAGO2精氨酸甲基化，抑制OsAGO2介导的基因沉默。我们的研究揭示了一个OsPRMT5-OsAGO2/miR1875-OsHXK1调控模块，它微调水稻对稻瘟病的防御反应。
Rice ARGONAUTE2 (OsAGO2) is a core component of the rice RNA‐induced silencing complex (RISC), which is repressed byMagnaporthe oryzae(M. oryzae) infection. Whether and how OsAGO2‐mediated gene silencing plays a role in rice blast resistance and which sRNAs participate in this process are unknown. Our results indicate that OsAGO2 is a key immune player that manipulates rice defense responses against blast disease. OsAGO2 associates with the 24‐nt miR1875 and binds to the promoter region ofHEXOKINASE1(OsHXK1), which causes DNA methylation and leads to gene silencing. Our multiple genetic evidence showed that, withoutM. oryzaeinfection, OsAGO2/miR1875 RISC promotedOsHXK1promoter DNA methylation andOsHXK1silencing; afterM. oryzaeinfection, the reduced OsAGO2/miR1875 led to a relatively activatedOsHXK1expression.OsHXK1acts as a positive regulator of blast disease resistance thatOsHXK1‐OErice exhibited enhanced resistance, whereasCas9‐Oshxk1rice showed reduced resistance againstM. oryzaeinfection. OsHXK1 may function through its sugar sensor activity as glucose induced defense‐related gene expression and reactive oxygen species (ROS) accumulation in Nipponbare andOsHXK1‐OEbut not inCas9‐Oshxk1rice. OsAGO2 itself is delicately regulated by OsPRMT5, which sensesM. oryzaeinfection and attenuates OsAGO2‐mediated gene silencing through OsAGO2 arginine methylation. Our study reveals an OsPRMT5‐OsAGO2/miR1875‐OsHXK1regulatory module that fine tunes the rice defense response to blast disease.