Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union.
Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union.
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DOI：
10.1155/2010/760819
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发表时间：
2010
影响因子：
3.4
通讯作者：
Sulakvelidze A
中科院分区：
文献类型：
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作者：
Rajanna C;Revazishvili T;Rashid MH;Chubinidze S;Bakanidze L;Tsanava S;Imnadze P;Bishop-Lilly KA;Sozhamannan S;Gibbons HS;Morris JG;Sulakvelidze A
测定了前苏联鼠疫耶尔森氏菌（Yersinia pestis，FSU）三株9.5kb pPCP 1质粒的全序列，并与已鉴定的非FSU鼠疫耶尔森氏菌（Yersinia pestis，FSU）三株9.5kb pPCP 1质粒的全序列进行了比较。鼠疫菌株（KIM、CO92和91001）。两个FSU质粒来自分离自俄罗斯鼠疫疫源地的菌株C2614和C2944，一个质粒来自吉尔吉斯斯坦的菌株C790。序列分析在6种质粒中鉴定出4种序列类型。FSU株中pPCP 1质粒与KIM株中pPCP 1质粒的遗传相关性最高，与Y株中pPCP 1质粒的遗传相关性最低。鼠疫91001。与菌株CO92相比，FSU菌株通常具有更大的pPCP1质粒拷贝数。菌株C2944中质粒pla基因的表达显著高于菌株CO92（P ≤ 0.05）。鉴于解放军在Y.鼠疫菌毒力，这种差异可能有重要意义的菌株的毒力。
Complete sequences of 9.5-kb pPCP1 plasmids in three Yersinia pestis strains from the former Soviet Union (FSU) were determined and compared with those of pPCP1 plasmids in three well-characterized, non-FSU Y. pestis strains (KIM, CO92, and 91001). Two of the FSU plasmids were from strains C2614 and C2944, isolated from plague foci in Russia, and one plasmid was from strain C790 from Kyrgyzstan. Sequence analyses identified four sequence types among the six plasmids. The pPCP1 plasmids in the FSU strains were most genetically related to the pPCP1 plasmid in the KIM strain and least related to the pPCP1 plasmid in Y. pestis 91001. The FSU strains generally had larger pPCP1 plasmid copy numbers compared to strain CO92. Expression of the plasmid's pla gene was significantly (P ≤ .05) higher in strain C2944 than in strain CO92. Given pla's role in Y. pestis virulence, this difference may have important implications for the strain's virulence.