In situ synthesis and biocompatibility of nano hydroxyapatite on pristine and chitosan functionalized graphene oxide
In situ synthesis and biocompatibility of nano hydroxyapatite on pristine and chitosan functionalized graphene oxide
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原始壳聚糖功能化氧化石墨烯上纳米羟基磷灰石的原位合成及其生物相容性
DOI：
10.1039/c2tb00053a
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Wei, Shicheng
中科院分区：
文献类型：
--
作者：
Li, Ming;Wang, Yanbo;Wei, Shicheng
受使用氧化石墨烯（GO）作为复合材料的纳米填料的成功的启发，有动力寻找这种新型的碳材料作为生物复合材料中的增强相。本文以氧化石墨烯和壳聚糖（CS）功能化氧化石墨烯为模板，采用溶液原位合成法制备了羟基磷灰石（HA），并首次成功制备了GO-HA和CS-GO-HA纳米复合材料。发现直径约27 +/- 7 nm、长度约150 +/- 25 nm的纺锤形HA纳米颗粒随机且强烈地装饰在原始和壳聚糖功能化GO基质的表面或聚集在其边缘。与HA相比，GO基HA纳米复合材料的弹性模量和硬度均有所提高。采用CCK-8法分别对小鼠成纤维细胞L-929和人成骨样细胞MG-63进行体外细胞毒性试验。两种纳米复合材料对L-929和MG-63均表现出高细胞增殖率，并且与GO-HA复合材料相比，CS-GO-HA可以提供显著更高的细胞活力和碱性磷酸酶活性。这些发现可能为GO基羟基磷灰石生物复合材料在骨修复、骨增强以及生物医学植入物涂层中的应用提供新的前景，并拓宽GO片在生物领域的应用。
Motivated by the success of using graphene oxide (GO) as a nanofiller of composites, there is a drive to search for this new kind of carbon material as a reinforcing phase in biocomposites. In the present work, graphene oxide and chitosan (CS) functionalized graphene oxide were introduced as templates to fabricate hydroxyapatite (HA) using a facile solution-based in situ synthesis method, and GO-HA and CS-GO-HA nanocomposites were successfully prepared for the first time. It was found that the spindle like HA nanoparticles with a diameter of about 27 +/- 7 nm and a length around 150 +/- 25 nm were decorated randomly and strongly on the surface or aggregated at the edges of the pristine and chitosan functionalized GO matrix. Compared with HA, the prepared GO-based HA nanocomposites displayed an increased elastic modulus and hardness. The in vitro cytotoxicity of the prepared nanocomposites was investigated using CCK-8 assay on murine fibroblast L-929 cell line and human osteoblast-like MG-63 cell line, respectively. Both of the nanocomposites exhibited a high cell proliferation rate for L-929 and MG-63, and the CS-GO-HA could provide significantly higher cell viability and alkaline phosphatase activity compared to the GO-HA composite. These findings may provide new prospects for utilizing the GO-based hydroxyapatite biocomposites in bone repair, bone augmentation, as well as coating of biomedical implants and broaden the application of GO sheets in biological areas.