SINGLE-CELL ORIGIN OF MOUSE HEMATOPOIETIC COLONIES EXPRESSING MULTIPLE LINEAGES IN VARIABLE COMBINATIONS
SINGLE-CELL ORIGIN OF MOUSE HEMATOPOIETIC COLONIES EXPRESSING MULTIPLE LINEAGES IN VARIABLE COMBINATIONS
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DOI：
10.1073/pnas.80.21.6689
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
OGAWA, M
中科院分区：
文献类型：
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作者：
SUDA, T;SUDA, J;OGAWA, M
通过使用显微操作器，将来自原始细胞集落的单个细胞单独转移到35 mm培养皿中进行二次集落形成。当单个集落似乎成熟时，通过May-Grunwald-Giemsa染色检查其细胞组成，并重新铺板以测定未表达的造血潜能。共描述了50个固定的造血集落。观察到由2种不同谱系的细胞组成的7种类型的集落，即，嗜酸性粒细胞-巨噬细胞、嗜酸性粒细胞-嗜酸性粒细胞、巨噬细胞-嗜酸性粒细胞、巨噬细胞-肥大细胞、巨噬细胞-巨核细胞、巨噬细胞-红细胞和红细胞-巨核细胞。6种类型的集落显示3种细胞谱系：嗜酸性粒细胞-巨噬细胞-嗜酸性粒细胞、嗜酸性粒细胞-巨噬细胞-肥大细胞、嗜酸性粒细胞-巨噬细胞-红细胞、巨噬细胞-肥大细胞-红细胞、嗜酸性粒细胞-巨噬细胞-巨核细胞和嗜酸性粒细胞-红细胞-巨核细胞。此外，存在以> 3个谱系的不同组合表达终末分化的多谱系集落。再铺板研究证实，这些集落中的许多的祖细胞仅在通过染色所揭示的谱系中终末致力于分化。本研究为表达不同细胞系组合的小鼠造血集落的单细胞起源提供了证据。多能造血祖细胞的分化显然是通过细胞谱系的渐进和随机限制。
By using a micromanipulator, single cells from blast cell colonies were individually transferred to 35-mm culture dishes for secondary colony formation. When individual colonies appeared to be mature, they were examined for cellular composition by May-Grunwald-Giemsa staining and were replated for determination of unexpressed hemopoietic potentials. A total of 50 fixed hemopoietic colonies were described. Seven types of colonies consisting of cells in 2 different lineages were seen, i.e., neutrophil-macrophage, neutrophil-eosinophil, macrophage-eosinophil, macrophage-mast cell, macrophage-megakaryocyte, macrophage-erythrocyte and erythrocyte-megakaryocyte. Six types of colonies revealed 3 cell lineages: neutrophil-macrophage-eosinophil, neutrophil-macrophage-mast cell, neutrophil-macrophage-erythrocyte, macrophage-mast cell-erythrocyte, neutrophil-macrophage-megakaryocyte and neutrophil-erythrocyte-megakaryocyte lineages. In addition, multilineage colonies expressing terminal differentiation in varying combinations of > 3 lineages were present. Replating studies confirmed that the progenitors for many of these colonies are terminally committed to differentiation only in the lineages disclosed by staining. This study provides a proof for the single-cell origin of mouse hemopoietic colonies expressing various combinations of cell lineages. The differentiation of multipotential hemopoietic progenitors apparently is through progressive and stochastic restriction in cell lineages.