A novel amphiphilic adsorbent for the removal of low-density lipoprotein.
A novel amphiphilic adsorbent for the removal of low-density lipoprotein.
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DOI：
10.1016/s0142-9612(03)00092-9
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发表时间：
2003-07
期刊：
影响因子：
14
通讯作者：
Shenqi Wang;Yao-ting Yu;T. Cui;Yan Cheng
中科院分区：
文献类型：
--
作者：
Shenqi Wang;Yao-ting Yu;T. Cui;Yan Cheng
通过以下步骤制备用于吸附低密度脂蛋白（LDL）的具有两亲性配体的葡聚糖吸附剂。胆固醇基团通过与胆固醇N-（6-异氰酸己基）氨基甲酸酯在吡啶存在下在DMSO中在80 ° C下反应而连接至右旋糖酐G 75。为了引入亲水部分，然后使其与氯磺酸在甲酰胺中反应，从而引入磺酸基。吸附剂对低密度脂蛋白胆固醇（LDL-C）、总胆固醇（TC）和甘油三酯（TG）的去除率分别为1.916、2.132和1.349mg/ml，对血浆总蛋白含量无明显影响。此外，该吸附剂对LDL-C、TC、TG的去除效果优于仅含疏水或亲水配体的葡聚糖。
Dextran adsorbent with amphiphilic ligands for adsorption of low-density lipoprotein (LDL) was prepared by the following procedures. Cholesterol group was linked to Dextran G 75 by reacting with cholesterol N-(6-isocyanatohexyl) carbamate in the presence of pyridine in DMSO at 80. In order to introduce the hydrophilic moiety, it was then reacted with chlorosulfonic acid in formamide which introduced the sulfonic group. Adsorption capacity of the adsorbent was studied which showed the removal of LDL-cholesterol (LDL-C), total cholesterol (TC) and TG to be 1.916, 2.132 and 1.349mg/ml, respectively without significantly affecting total protein levels in the plasma. Moreover, the adsorbent has a better selectivity in removing LDL-C, TC, TG compared to dextran which has only hydrophobic or hydrophilic ligand.