Sustained and Extended Release with Structural and Activity Recovery of Lysozyme from Complexes with Sodium (Sulfamate Carboxylate) Isoprene/Ethylene Oxide Block Copolymer
Sustained and Extended Release with Structural and Activity Recovery of Lysozyme from Complexes with Sodium (Sulfamate Carboxylate) Isoprene/Ethylene Oxide Block Copolymer
复制标题
从（氨基磺酸钠）异戊二烯/环氧乙烷嵌段共聚物复合物中恢复溶菌酶的结构和活性，持续和延长释放
DOI：
10.1002/mabi.200900186
复制
发表时间：
2010-02-11
影响因子：
4.6
通讯作者：
Yao, Ping
中科院分区：
文献类型：
--
作者：
Gao, Gao;Yan, Yunfeng;Yao, Ping
研究了溶菌酶与异戊二烯/环氧乙烷（SCIEO）钠盐在pH = 7.4条件下的络合作用以及在NaCl存在下溶菌酶从络合物中的释放。通过静电和疏水相互作用，溶菌酶和SCIEO形成稳定的复合纳米颗粒。这种络合作用部分干扰了溶菌酶的结构。溶菌酶的一些疏水残基被暴露以与SCIEO结合。络合导致大部分溶菌酶活性的损失。在氯化钠存在下，复合物中可以释放出溶菌酶。释放的溶菌酶分子完全恢复其天然结构和活性。在生理pH和离子强度条件A下，溶菌酶的释放达到了缓释和延长。
The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residues of lysozyme are exposed to bind with SCIEO. The complexation leads to loss of most of the lysozyme activity. In the presence of NaCl, lysozyme can be released from the complexes. The released lysozyme molecules recover their native structure and activity completely. In the condition A of physiological pH and ionic strength, a sustained and extended release of lysozyme was achieved.