Molecular re-examination of the taxonomy of the Meligethes viridescens species complex (Coleoptera: Nitidulidae)
Molecular re-examination of the taxonomy of the Meligethes viridescens species complex (Coleoptera: Nitidulidae)
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DOI：
10.1016/s0305-1978(99)00039-3
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发表时间：
2000-01-01
影响因子：
1.6
通讯作者：
De Matthaeis, E
中科院分区：
文献类型：
--
作者：
Audisio, P;De Biase, A;De Matthaeis, E
利用等位酶和RAPD技术分析了绿盲蝽复合群（鞘翅目，绿盲蝽科，盲蝽亚科）的亲缘关系。来自意大利中部的5个居群分属3个种：M. viridescens、绿枝霉M. czwalinai和一新种（M，sp. cfr. viridescens）和形态分化良好的M. Aeneus进行了分析。观察到的遗传分化程度支持分离在特定级别的M。viridescens，M. czwalinai和M. sp. cfr. viridescens。基于扫描电子显微镜的物种生态学和形态学观察也支持这样的分类分离。RAPD引物M13可作为鉴别这4个种的诊断标记。从等位酶遗传距离数据计算的分歧时间的估计表明，观察到的分化模式可能与古气候变化在欧洲南部的上新世/更新世冰周期。(C)1999由Elsevier Science Ltd.出版。保留所有权利。
Allozyme and RAPD analysis were performed to clarify taxonomic relationships within the Meligethes viridescens complex (Coleoptera, Nitidulidae, Meligethinae). Five populations from Central Italy belonging to three species: M. viridescens, M. czwalinai, and a new undescribed species (M, sp. cfr. viridescens), and one population of the morphologically well-differentiated M. aeneus were analysed. The degree of observed genetic differentiation supports separation at the specific rank of M. viridescens, M, czwalinai, and M. sp. cfr. viridescens. The species' ecology and morphological observations based on scanning electron microscopy also supports such a taxonomic separation. The M13 RAPD primer is a diagnostic marker for distinguishing the four species. Estimates of divergence times calculated from allozymic genetic distance data suggest that the pattern of differentiation observed may be related to the palaeoclimatic changes during the Plio/Pleistocene ice cycles in southern Europe. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.