Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain
Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain
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DOI：
10.1016/j.vetmic.2015.06.015
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发表时间：
2015-09-30
影响因子：
3.3
通讯作者：
Tong, Guang-zhi
中科院分区：
文献类型：
--
作者：
Jiang, Yi-feng;Xia, Tian-qi;Tong, Guang-zhi
从江苏省中国猪场3头死猪中分离到3株猪繁殖与呼吸综合征病毒，分别为NT1、NT2和NT3。全基因组序列分析表明，这三个分离株与高致病性PRRSV JXA1在Marc-145细胞中连续传代形成的弱毒疫苗株JXA1-P80同源性最高。在在MARC-145细胞上弱化的JXA1的ORF1a、ORF1b、GP4和GP5中，分别在NT1、NT2和NT3的相应位置发现了10多个氨基酸残基。在毒力分析中，感染NT1、NT2或NT3的仔猪表现出疾病的临床症状，包括高烧、厌食和呼吸窘迫，导致大多数仔猪在两周内死亡。总体而言，这些数据表明NT1、NT2和NT3是高致病性PRRSVs，它们很可能是疫苗株JXA1-P80的回复变种。(C)2015爱思唯尔B.V.保留所有权利。
Three porcine reproductive and respiratory syndrome viruses (PRRSV), NT1, NT2, and NT3, were isolated from three dying piglets from a single pig farm in Jiangsu Province, China. Whole genome sequencing revealed that the three isolates share the highest homology with JXA1-P80, an attenuated vaccine strain developed by serial passage of highly pathogenic PRRSV JXA1 in MARC-145 cells. More than ten amino acids residues in ORF1a, ORF1b, GP4, and GP5 that were thought to be unique to JXA1 attenuated on MARC-145 cells were each found in the corresponding locations of NT1, NT2, and NT3. In virulence assays, piglets infected with NT1, NT2, or NT3 exhibited clinical signs of disease, including high fever, anorexia, and respiratory distress, leading to the death of the majority of the piglets within two weeks. Collectively, these data indicate that NT1, NT2, and NT3 are highly pathogenic PRRSVs and they are likely to be revertants of the vaccine strain JXA1-P80. (C) 2015 Elsevier B.V. All rights reserved.