Relationship between in vivo age and in vitro aging: assessment of 669 cell cultures derived from members of the Baltimore Longitudinal Study of Aging.
Relationship between in vivo age and in vitro aging: assessment of 669 cell cultures derived from members of the Baltimore Longitudinal Study of Aging.
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体内年龄和体外衰老之间的关系：对来自巴尔的摩纵向衰老研究成员的 669 个细胞培养物进行评估。
DOI：
10.1093/gerona/57.6.b239
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Schneider,EdwardL
中科院分区：
文献类型：
--
作者：
Smith,JamesR;Venable,Susan;Roberts,ThomasW;Metter,EJeffrey;Monticone,Robert;Schneider,EdwardL
We examined the in vitro proliferative potential of 669 cell cultures established from skin biopsies of members of the Baltimore Longitudinal Study of Aging. The colony size distribution was used to estimate the proliferative life span of the cultures. A significant decline in proliferative potential with donor age was observed for female but not male donors. For both male and female donors, the proliferative potential was significantly greater for donors under the age of 30 years compared with all donors over the age of 30 years. In an attempt to reduce genetic heterogeneity, we examined the proliferative potential of cultures derived at different ages from the same donor. These studies revealed a trend (approaching statistical significance) toward low proliferative potential as donors aged. Interestingly, samples obtained from donors who had a history of skin cancer at the time of biopsy had a significantly lower doubling potential than those from donors who did not. The implications of these results for the use of cells derived from donors of different ages for aging research are discussed.
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来自老年 Fischer 344 大鼠的皮肤成纤维细胞在复制寿命方面经历了类似的变化，但由于供体的热量限制而没有永生化。
影响因子：
3.7
作者：
R. Pignolo;E. Masoro;W. W. Nichols;C. Bradt;V. Cristofalo
通讯作者：
V. Cristofalo
DOI：
10.1073/pnas.95.18.10614
发表时间：
1998-09-01
影响因子：
11.1
作者：
Cristofalo, VJ;Allen, RG;Beck, JC
通讯作者：
Beck, JC
MRC-5 人成纤维细胞体外老化过程中的染色体变化。
影响因子：
3.7
作者：
K. Thompson;R. Holliday
通讯作者：
R. Holliday
体外细胞衰老与体内人类年龄之间的关系。
DOI：
--
发表时间：
1976
影响因子：
11.1
作者：
E. Schneider;Y. Mitsui
通讯作者：
Y. Mitsui
糖尿病和遗传性糖尿病前期。
DOI：
10.1172/jci109311
发表时间：
1979
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
S. Goldstein;E. Moerman;J. Soeldner;R. Gleason;D. M. Barnett
通讯作者：
D. M. Barnett