Noninvasive Monitoring of Glioma Growth in the Mouse.
Noninvasive Monitoring of Glioma Growth in the Mouse.
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DOI：
10.7150/jca.15564
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发表时间：
2016
影响因子：
3.9
通讯作者：
Malatesta P
中科院分区：
文献类型：
--
作者：
Alessandrini F;Ceresa D;Appolloni I;Marubbi D;Malatesta P
恶性胶质瘤是最常见和最致命的原发性恶性脑肿瘤。体内原位模型无疑可以代表一个合适的工具，以测试新的治疗胶质瘤。然而，通常用于监测小鼠脑内胶质瘤生长的方法是耗时和侵入性的。我们测试了基于分泌的荧光素酶（Gaussia荧光素酶）的微创手术的可靠性，以频繁监测胶质瘤大小的变化。每周两次从小鼠尾尖收集的血液样品评估Gluc活性，从而制作肿瘤的生长曲线。我们通过分析脑解剖后的肿瘤来验证Gluc活性与肿瘤大小之间的相关性。我们发现，这种方法是可靠的监测移植在免疫缺陷小鼠的人脑胶质瘤，但它有很强的局限性，在免疫活性模型，对荧光素酶的免疫反应是在移植后的第一周内开发。
Malignant gliomas are the most common and deadly primary malignant brain tumors. In vivo orthotopic models could doubtless represent an appropriate tool to test novel treatment for gliomas. However, methods commonly used to monitor the growth of glioma inside the mouse brain are time consuming and invasive. We tested the reliability of a minimally invasive procedure, based on a secreted luciferase (Gaussia luciferase), to frequently monitor the changes of glioma size. Gluc activity was evaluated from blood samples collected from the tail tip of mice twice a week, allowing to make a growth curve for the tumors. We validated the correlation between Gluc activity and tumor size by analysing the tumor after brain dissection. We found that this method is reliable for monitoring human glioma transplanted in immunodeficient mice, but it has strong limitation in immunocompetent models, where an immune response against the luciferase is developed during the first weeks after transplant.
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影响因子：
3.7
作者：
Appolloni, Irene;Calzolari, Filippo;Malatesta, Paolo
通讯作者：
Malatesta, Paolo
影响因子：
3.7
作者：
Badr CE;Hewett JW;Breakefield XO;Tannous BA
通讯作者：
Tannous BA
影响因子：
2.9
作者：
Branchini, BR;Southworth, TL;Roda, A
通讯作者：
Roda, A
DOI：
10.1007/978-1-62703-718-1_17
发表时间：
2014-01-01
期刊：
BIOLUMINESCENT IMAGING: METHODS AND PROTOCOLS
影响因子：
--
作者：
Lewandrowski, Grant K.;Magee, Ciara N.;Azzi, Jamil
通讯作者：
Azzi, Jamil
影响因子：
5.2
作者：
Tseng, Alan Wei-Shun;Akerstrom, Victoria;Lan, Michael S.
通讯作者：
Lan, Michael S.