Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene
Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene
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DOI：
10.1038/nature02538
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发表时间：
2004-06-03
期刊：
影响因子：
64.8
通讯作者：
Winston, F
中科院分区：
文献类型：
--
作者：
Martens, JA;Laprade, L;Winston, F
在酿酒酵母和人类中，RNA聚合酶II的转录是广泛的，甚至在不编码蛋白质的基因组区域中也是如此（1-6）。这种基因间转录的目的在很大程度上是未知的，尽管它可以是调节性的（7，8）。我们通过对S. cerevisiae SER 3基因。我们之前的结果表明，在丰富培养基中生长期间，SER 3的转录受到严格抑制（9）。我们现在表明，该基因的调控区在这些条件下高度转录，并产生非蛋白质编码RNA（SRG 1）。SRG 1 RNA的表达是抑制SER 3所必需的。另外的实验已经证明，阻遏通过转录干扰机制发生，其中SRG 1跨SER 3启动子的转录干扰激活剂的结合。这项工作确定了一个以前未知的一类转录调控基因。
Transcription by RNA polymerase II in Saccharomyces cerevisiae and in humans is widespread, even in genomic regions that do not encode proteins(1-6). The purpose of such intergenic transcription is largely unknown, although it can be regulatory(7,8). We have discovered a role for one case of intergenic transcription by studying the S. cerevisiae SER3 gene. Our previous results demonstrated that transcription of SER3 is tightly repressed during growth in rich medium(9). We now show that the regulatory region of this gene is highly transcribed under these conditions and produces a non-protein-coding RNA (SRG1). Expression of the SRG1 RNA is required for repression of SER3. Additional experiments have demonstrated that repression occurs by a transcription-interference mechanism in which SRG1 transcription across the SER3 promoter interferes with the binding of activators. This work identifies a previously unknown class of transcriptional regulatory genes.