NagR(Bt) Is a Pleiotropic and Dual Transcriptional Regulator in Bacillus thuringiensis.
NagR(Bt) Is a Pleiotropic and Dual Transcriptional Regulator in Bacillus thuringiensis.
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NagRBt 是苏云金芽孢杆菌中的多效性和双重转录调节因子。
DOI：
10.3389/fmicb.2018.01899
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发表时间：
2018
影响因子：
5.2
通讯作者：
Cai J
中科院分区：
文献类型：
--
作者：
Cao ZL;Tan TT;Zhang YL;Han L;Hou XY;Ma HY;Cai J
NagR属于GntR/HutC家族，是一种负调节因子，直接抑制nagP和nagAB基因，这两个基因参与枯草芽孢杆菌中GlcNAc的转运和利用。我们之前的工作证实，苏云金芽孢杆菌菌株 Bti75 的几丁质酶 B 基因（chiB）也受到枯草芽孢杆菌 NagR 的直系同源物 YvoABt 的负控制。在这项工作中，我们研究了 Bti75 中的调节网络，发现 YvoABt 是一种 N-乙酰氨基葡萄糖利用调节剂，主要参与 GlcNAc 分解代谢；因此 YvoABt 更名为 NagRBt。 RNA-seq数据显示，与野生型菌株相比，ΔnagR突变体中有27个基因上调，14个基因下调。通过 RNA-seq、生物信息学软件、电泳迁移率变动分析和定量实时逆转录 PCR 来表征调节子（指数期）。在Bti75基因组中，鉴定出19个受NagRBt直接调控的基因和30个受NagRBt间接调控的基因。我们编制的计算机、体外和体内证据表明，NagRBt 作为阻遏剂和激活剂，直接或间接影响涉及氨基糖代谢、核苷酸代谢、脂肪酸代谢、磷酸转移酶系统和 Embden-Meyerhof-Parnas 途径的主要生物过程。
NagR, belonging to the GntR/HutC family, is a negative regulator that directly represses the nagP and nagAB genes, which are involved in GlcNAc transport and utilization in Bacillus subtilis. Our previous work confirmed that the chitinase B gene (chiB) of Bacillus thuringiensis strain Bti75 is also negatively controlled by YvoABt, the ortholog of NagR from B. subtilis. In this work, we investigated its regulatory network in Bti75 and found that YvoABt is an N-acetylglucosamine utilization regulator primarily involved in GlcNAc catabolism; therefore YvoABt is renamed as NagRBt. The RNA-seq data revealed that 27 genes were upregulated and 14 genes were downregulated in the ΔnagR mutant compared with the wild-type strain. The regulon (exponential phase) was characterized by RNA-seq, bioinformatics software, electrophoretic mobility shift assays, and quantitative real-time reverse transcription PCR. In the Bti75 genome, 19 genes that were directly regulated and 30 genes that were indirectly regulated by NagRBt were identified. We compiled in silico, in vitro, and in vivo evidence that NagRBt behaves as a repressor and activator to directly or indirectly influence major biological processes involved in amino sugar metabolism, nucleotide metabolism, fatty acid metabolism, phosphotransferase system, and the Embden–Meyerhof–Parnas pathway.
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影响因子：
4.5
作者：
Langdon WB
通讯作者：
Langdon WB
影响因子：
9.8
作者：
Li XY;MacArthur S;Bourgon R;Nix D;Pollard DA;Iyer VN;Hechmer A;Simirenko L;Stapleton M;Luengo Hendriks CL;Chu HC;Ogawa N;Inwood W;Sementchenko V;Beaton A;Weiszmann R;Celniker SE;Knowles DW;Gingeras T;Speed TP;Eisen MB;Biggin MD
通讯作者：
Biggin MD
影响因子：
3.6
作者：
Komatsuzawa, H;Fujiwara, T;Sugai, M
通讯作者：
Sugai, M
DOI：
10.1128/genomea.01023-13
发表时间：
2013-12-26
期刊：
Genome announcements
影响因子：
--
作者：
Doggett NA;Stubben CJ;Chertkov O;Bruce DC;Detter JC;Johnson SL;Han CS
通讯作者：
Han CS
DOI：
10.1073/pnas.0506687102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Grainger, DC;Hurd, D;Busby, SJW
通讯作者：
Busby, SJW