CLONING OF NORMAL MAST CELLS IN TISSUE CULTURE
CLONING OF NORMAL MAST CELLS IN TISSUE CULTURE
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DOI：
10.1002/jcp.1030660309
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发表时间：
1965-01-01
影响因子：
--
通讯作者：
SACHS, L
中科院分区：
文献类型：
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作者：
PLUZNIK, DH;SACHS, L
描述了在软琼脂培养基中组织培养正常小鼠“肥大”细胞的克隆方法。菌落中含有不同分化阶段的细胞。结果表明，单个集落形成单元可以形成一个集落，并且集落的形成是细胞增殖的结果。成人脾细胞悬液每105个细胞可产生3个菌落。这些菌落的再克隆每103个细胞约有3个菌落。成年小鼠脾脏的菌落频率高于成年胸腺。在成人淋巴结细胞中没有获得这样的集落。菌落的形成需要胚胎细胞饲养层的存在。由于饲养层是在琼脂下面播种的，结果表明，“肥大”细胞生长和分化所需的物质可以通过琼脂。
A method is described for the cloning of normal mouse “mast” cells in tissue culture in a soft agar medium. The colonies contain cells in different stages of differentiation. It was shown that a colony can be initiated by a single colony forming unit, and that colonies are formed as a result of cell multiplication.Cell suspensions from adult spleen gave about 3 colonies per 105cells seeded. A re‐cloning of these colonies gave about 3 colonies per 103cells seeded.The frequency of colonies from SWR mice was higher with adult spleen than with adult thymus. No such colonies were obtained with adult lymph node cells.The formation of colonies was shown to require the presence of an embryo cell feeder layer. Since the feeder layers were seeded underneath the agar, the results indicate that the substance(s) required for the growth and differentiation of “mast” cells can pass through agar.