Effects of enteric neural stimulation on chloride transport in human left colon in vitro.
Effects of enteric neural stimulation on chloride transport in human left colon in vitro.
复制标题
肠神经刺激对体外人左结肠氯转运的影响。
DOI：
10.1007/bf01536052
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
McGregor,B
中科院分区：
文献类型：
--
作者：
Kuwahara,A;Cooke,HJ;Carey,HV;Mekhjian,H;Ellison,EC;McGregor,B
非病理性段肌肉剥离左结肠的病人接受手术的癌症，憩室炎，或其他原因，建立在通量室，以确定内在神经元对离子转运的影响。在基础状态下，钠和氯都被积极吸收，并且没有显著的残留离子通量。电场刺激的内在粘膜神经支配引起的短路电流，这是依赖于刺激频率和强度的增加。这种反应被蝎子毒液模仿，蝎子毒液被认为是去神经元的，而河豚毒素几乎被消除，河豚毒素阻止了神经递质的释放。阿托品减少，但没有取消，对神经刺激的反应。卡巴胆碱，氨茶碱，茶碱显着增加短路电流高于基础水平。电场刺激引起的短路电流的增加，可以解释为净氯离子吸收的减少，而没有任何显着的影响钠吸收或残留离子通量。这些结果表明，在人类左结肠的离子转运的内在粘膜下胆碱能神经元以及其他神经元类型的调节，这些神经的激活可以提供某些神经系统疾病的基础。
Nonpathological segments of muscle-stripped left colon from patients undergoing surgery for carcinoma, diverticulitis, or other causes were set up in flux chambers in order to determine the influence of intrinsic neurons on ion transport. In the basal state, both sodium and chloride were actively absorbed, and there was no significant residual ion flux. Electrical field stimulation of the intrinsic mucosal innervation evoked an increase in short-circuit current that was dependent on stimulus frequency and strength. The response was mimicked by scorpion venom, which is known to depolarize neurons, and was nearly abolished by tetrodotoxin, which prevents neurotransmitter release. Atropine reduced, but did not abolish, the response to neural stimulation. Carbachol, aminophylline, and theophylline significantly increased short-circuit current above basal levels. Electrical field stimulation evoked an increase in short-circuit current that could be accounted for by a decrease in net chloride absorption without any significant effect on sodium absorption or residual ion flux. These results suggest that ion transport in the human left colon is regulated by intrinsic submucosal cholinergic neurons as well as other neuronal types, and activation of these nerves could provide the basis for certain diarrheal disorders.