The Role of Breast Cancer Stem Cells and Some Related Molecular Biomarkers in Metastatic and Nonmetastatic Breast Cancer
The Role of Breast Cancer Stem Cells and Some Related Molecular Biomarkers in Metastatic and Nonmetastatic Breast Cancer
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DOI：
10.1016/j.clbc.2019.11.008
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发表时间：
2020-08-01
影响因子：
3.1
通讯作者：
Bahnassy, Abeer A.
中科院分区：
文献类型：
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作者：
Abu El Abbass, Khlood;Abdellateif, Mona S.;Bahnassy, Abeer A.
这项工作旨在评估BCSC标志物表达的作用和来自44名患有IDC的埃及妇女和25名接受乳房缩小术的健康妇女作为对照的BC组织培养物中乳房球的数量。背景：乳腺癌干细胞（Breast cancer stem cells，BCSCs）在浸润性导管癌（Invasive duct carcinoma，IDC）的发生、发展中起重要作用。我们评估了BCSC标志物表达的作用和乳腺癌（BC）组织培养物中乳腺球的数量，并将这些数据与患者的相关临床病理特征和总生存期（OS）相关联。研究方法：从44名患有乳腺IDC的埃及女性患者和25名接受乳房缩小术的健康女性中收集新鲜肿瘤组织样本作为对照。在BCSC分化的不同阶段，与对照样品相比，通过逆转录聚合酶链反应评估乳腺球数量和一些癌症干细胞相关基因（PTEN、PI 3 K、AKT、Wnt和β-连环蛋白）的RNA表达水平。结果如下：培养结束时CD 44（+）CD 24（-）（/low）细胞数与Wnt、β-catenin表达水平和远处转移显著相关（分别为P <0.001、P = 0.015和P = 0.003）。乳腺球数量与CD 44（+）CD 24（-）（/低）细胞以及AKT表达之间存在显著相关性（分别为P = .040和.021）。PTEN mRNA在BC中的表达显著高于对照组（P <0.05）。Wnt-RNA表达与高肿瘤分期、阳性淋巴结状态、Her 2-neu过表达和转移显著相关（分别为P = .009、.012、.026和.001），而OS与远处转移、Wnt和PTEN表达显著相关（分别为P < .001、P = .001、P = .014）。结论：BCSC及其相关基因（PTEN、PI 3 K、AKT、Wnt和β-catenin）在BC的发生和进展中起重要作用，它们可用作BC患者的潜在预后和预测生物标志物或作为靶向治疗。(C)2019爱思唯尔公司All rights reserved.
This work was designed to assess the role of BCSC marker expression and the number of mammospheres in cultures of BC tissues from 44 Egyptian women with IDC and 25 healthy women undergoing reduction mammoplasty as controls. BCSCs and their related genes can be used as potential prognostic and predictive biomarkers for patients with BC as target therapy.Background: Breast cancer stem cells (BCSCs) play important role(s) in the development and progression of invasive duct carcinoma (IDC). We assessed the role of BCSC marker expression and the number of mammospheres in cultures of breast cancer (BC) tissues and correlated these data to relevant clinicopathologic features of the patients and overall survival (OS). Methods: Fresh tumor tissue samples were collected from 44 Egyptian female patients with IDC of the breast and 25 healthy women undergoing reduction mammoplasty as a control. The mammosphere number and the RNA expression levels of some cancer stem cell-related genes (PTEN, PI3K, AKT, Wnt, and beta-catenin) were assessed by reverse-transcriptase polymerase chain reaction at different stages of BCSC differentiation compared with control samples. Results: The number of CD44(+)CD24(-)(/low) cells associated significantly at the end of culture with the expression level of Wnt, beta-catenin, and distant metastasis (P < .001, P = .015 and P = .003, respectively). There was significant association between the mammosphere number and CD44(+)CD24(-)(/low) cells as well as AKT expression (P = .040 and .021, respectively). PTEN messenger RNA expressed significantly in BC (P < .05). Wnt-RNA expression associated significantly with high tumor stage, positive lymph node status, Her2-neu overexpression, and metastasis (P = .009, .012, .026, and .001, respectively), whereas OS associated significantly with distant metastasis, Wnt, and PTEN expressions (P < .001, P = .001, P = .014, respectively). Conclusion: BCSCs and their related genes (PTEN, PI3K, AKT, Wnt, and beta-catenin) play important roles in the development and progression of BC and they can be used as potential prognostic and predictive biomarkers for patients with BC or as target therapy. (C) 2019 Elsevier Inc. All rights reserved.