A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product
A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product
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DOI：
10.1006/bbrc.2001.5359
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发表时间：
2001-08-17
影响因子：
3.1
通讯作者：
Zhang, Y
中科院分区：
文献类型：
--
作者：
Lai, R;Liu, H;Zhang, Y
两栖动物皮肤含有丰富的缓激肽相关肽，但这些肽的生物合成方式尚不清楚。在本研究中，一种新型缓激肽相关肽，称为铃蟾激肽M，从中国红腹铃蟾（Bombina maxima）的皮肤分泌物中纯化得到。其一级序列确定为DLPKINRKGPRPPGFSPFR，它包含缓激肽从其N端延伸出的一个由10个残基组成的片段DLPKINRKGP。发现铃蟾激肽M的cDNA结构包含一个624个核苷酸的编码区。所编码的铃蟾激肽M前体由一个信号肽、一个酸性肽、六个100%相同的28个氨基酸的肽单元（包括铃蟾激肽M）以及一个间隔肽组成。铃蟾激肽M的序列之前有一个单一的碱性残基（精氨酸），它代表了释放成熟铃蟾激肽M的切割位点。这是首次从两栖动物皮肤中克隆缓激肽相关肽的cDNA。编码铃蟾激肽M的独特cDNA结构表明，两栖动物皮肤和哺乳动物血液系统中缓激肽相关肽的产生方式是不同的。（C）2001学术出版社
Amphibian skin contains rich bradykinin-related peptides, but the mode of biosynthesis of these peptides is unknown. In the present study, a novel bradykinin-related peptide, termed bombinakinin M, was purified from skin secretions of the Chinese red belly toad Bombina maxima. Its primary sequence was established as DLPKINRKGPRPPGFSPFR that comprises bradykinin extended from its N-terminus by a 10-residue segment DLPKINRKGP. The cDNA structure of bombinakinin M was found to contain a coding region of 624 nucleotides. The encoded precursor of bombinakinin M is composed of a signal peptide, an acidic peptide, six 100% identical copies of a 28-amino-acid peptide unit including bombinakinin M plus a spacer peptide. The sequence of bombinakinin M is preceded by a single basic residue (arginine), which represents the site of cleavage for releasing of mature bombinakinin M. This is the first cDNA cloning of bradykinin-related peptides from amphibian skin. The unique cDNA structure encoding bombinakinin M suggests that the generation modes of bradykinin-related peptides in amphibian skin and in mammalian blood system are different. (C) 2001 Academic Press.