Expression profiles and prognostic roles of m6A writers, erasers and readers in gastric cancer
Expression profiles and prognostic roles of m6A writers, erasers and readers in gastric cancer
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m6A 写入器、擦除器和读取器在胃癌中的表达谱和预后作用
DOI：
10.2217/fon-2020-0630
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发表时间：
2021-04-21
期刊：
影响因子：
3.3
通讯作者：
Yuan, Yuan
中科院分区：
文献类型：
--
作者：
Jing, Jing-Jing;Zhao, Xu;Yuan, Yuan
目的：探讨N6-甲基腺苷（m6 A）酶（书写者、擦除者和阅读者）的表达谱及其与胃癌（GC）预后的关系。方法：利用UALCAN和Oncomine网络资源分析基因表达。这些基因在GC中的预后作用是使用癌症基因组图谱的数据进行分析的。结果：13种m6 A酶在胃癌组织中表达上调。m6 A writers胃黏膜L3、RBM 15和WTAP的表达与病理分期有关。m6 A擦除剂FTO与肿瘤分期有关，ALKBH 5表达与胃癌预后有关。m6 A阅读器YTHDF 3表达与肿瘤分期相关。YTHDC 2与胃癌患者的生存率相关。结论：m6 A RNA甲基化关键基因的异常改变可能对胃癌的发生和预后有重要影响。我们的目的是探讨这些m6 A酶的表达谱及其与胃癌（GC）的发展和预后的关系。使用UALCAN和Oncomine网络资源分析基因表达。临床数据从癌症基因组图谱下载。利用STRING和GeneMANIA软件构建了基因-基因网络和蛋白质-蛋白质相互作用网络。发现13种m6 A酶在GC组织中上调。m6 A writers胃黏膜L3、RBM 15和WTAP的表达与病理分期有关。m6 A擦除剂FTO与肿瘤分期有关，ALKBH 5表达与胃癌预后有关。m6 A阅读器YTHDF 3表达与肿瘤分期相关，YTHDC 2与胃癌患者的生存相关。这些结果提示，参与m6 A RNA甲基化调控和功能的关键基因的异常变化可能对GC的发生和临床预后有重要影响。
Aim: To explore the expression profiles of N6-methyladenosine (m6A) enzymes (writers, erasers and readers) and their associations with gastric cancer (GC) prognosis. Methods: Gene expression was analyzed using the UALCAN and Oncomine web resources. The prognostic roles of these genes in GC were analyzed using data from The Cancer Genome Atlas. Results: Thirteen m6A enzymes were found to be upregulated in GC tissues. The expression of m6A writers METTL3, RBM15 and WTAP was associated with pathological stage. The m6A eraser FTO was related to tumor stage and ALKBH5 expression was related to GC prognosis. The m6A reader YTHDF3 expression was associated with tumor stage. YTHDC2 was associated with survival of GC patients. Conclusion: Abnormal changes of key genes involved in m6A RNA methylation may have an important impact on GC development and prognosis.Lay abstractRNA N6-methyladenosine (m6A) methylation is modulated by a series of epigenetic modulator enzymes termed 'writers', 'erasers' and 'readers'. We aimed to explore the expression profiles of these m6A enzymes and their associations with gastric cancer (GC) development and prognosis. Gene expression was analyzed using the UALCAN and Oncomine web resources. Clinical data were downloaded from The Cancer Genome Atlas. Gene-gene networks and protein-protein interaction networks were built using the softwares STRING and GeneMANIA. Thirteen m6A enzymes were found to be upregulated in GC tissues. The expression of m6A writers METTL3, RBM15 and WTAP was associated with pathological stage. The m6A eraser FTO was related to tumor stage and ALKBH5 expression was related to GC prognosis. The m6A reader YTHDF3 expression was associated with tumor stage, and YTHDC2 was associated with survival of GC patients. In summary, these results suggest that abnormal changes of key genes involved in regulation and function of m6A RNA methylation may have an important impact on GC development and its clinical prognosis.