Structural characterization of polysaccharides from Hyriopsis cumingii
Structural characterization of polysaccharides from Hyriopsis cumingii
复制标题
三角帆蚌多糖的结构表征
DOI：
10.1016/j.carbpol.2010.06.048
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发表时间：
2010-11-11
影响因子：
11.2
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
--
作者：
Qiao, Deliang;Liu, Jun;Zeng, Xiaoxiong
采用高碘酸氧化法、Smith降解法对纯化的三角帆蚌多糖（HCPS）进行结构表征。甲基化结合HPLC、GC、GC-MS、FUR和NMR谱。结果表明，纯化的HCPS糖环为吡喃糖环。在HCPS-1和HCPS-2中，糖基主要通过α-构型糖苷键连接。关于HCPS-3糖残基通过α-和β-构型糖苷键连接。HCPS-1、HCPS-2和HCPS-3的每个重复单元中的糖基残基数分别为7、9和10。纯化的HCPS的主链通过1 -> 4个糖苷键连接。在HCPS-1和HCPS-2中，一个重复单元中有一条主链和两条分支链（一条由1 -> 3糖苷键连接，另一条由1 -> 6糖苷键连接）。对于HCPS-3，在一个重复单元中，三个分支链（两个通过1 -> 3糖苷键连接，一个通过1 -> 6糖苷键连接）连接在主链上。(C)2010爱思唯尔有限公司保留所有权利。
The structural characterization of purified Hyriopsis cumingii polysaccharides (HCPS) were studied by using periodic acid oxidation, Smith degradation. methylation combined with HPLC, GC, GC-MS, FUR and NMR spectra. Results showed that sugar rings of purified HCPS were pyranose rings. In HCPS-1 and HCPS-2, glycosyl residues were linked mainly by a-configuration glycosidic bond. As to HCPS-3. sugar residues were connected by alpha- and beta-configuration glycosidic bonds. Number of glycosyl residues in one repeat unit was 7, 9 and 10 for HCPS-1, HCPS-2 and HCPS-3 respectively. Backbone chains of purified HCPS were linked by 1 -> 4 glycosidic bonds. In HCPS-1 and HCPS-2, there were one backbone chain and two branch chains (one linked by 1 -> 3 glycosidic bond, another connected by 1 -> 6 glycosidic bond) in one repeat unit. As to HCPS-3, three branch chains (two connected by 1 -> 3 glycosidic bond, one linked by 1 -> 6 glycosidic bond) were attached to backbone chain in one repeat unit. (C) 2010 Elsevier Ltd. All rights reserved.