Spleen atrophy related immune system changes attributed to infection of Angiostrongylus cantonensis in mouse model
Spleen atrophy related immune system changes attributed to infection of Angiostrongylus cantonensis in mouse model
复制标题
广州管圆线虫感染小鼠模型中脾萎缩相关免疫系统变化
DOI：
10.1007/s00436-016-5322-9
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发表时间：
2017-02-01
影响因子：
2
通讯作者：
Wu, Zhong-Dao
中科院分区：
文献类型：
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作者：
Liu, Zhen;Wu, Yu;Wu, Zhong-Dao
脾脏是重要的外周免疫器官之一，在感染性疾病中是最常见的受累器官。感染性疾病可引起脾脏的改变，包括脾萎缩和脾功能改变，而脾萎缩又可能影响感染性疾病的恢复。广州管圆线虫病是由广州管圆线虫（A. cantonensis），其侵入非允许宿主，如人和小鼠，引起对中枢神经系统（CNS）的严重损伤和急性炎症反应。a.已证实，广州线虫感染诱导的CNS损伤是由于源自外周免疫组分的深刻的免疫病理学。然而，免疫病理学的机制在很大程度上仍然未知。在这里，我们发现A.广州线虫只侵入非许可宿主如小鼠的大脑，但不侵入其他外周器官。然而，这种感染引起严重的脾萎缩。我们进一步认识到，这种萎缩与总脾细胞数量减少和脾结构破坏有关，这是由于增殖减少和脾细胞增多所致。外周血T细胞亚群下降，CD4/CD8比值和B/T细胞比值降低，脾脏CD4 + CD25 + Foxp3 + Treg、CD8 + CD28-T和CD38 + T淋巴细胞比值升高。阿苯达唑治疗可在一定程度上缓解脾脏萎缩，促进T细胞免疫重建。我们的数据表明A.广州杆菌感染可导致脾脏萎缩。这些结果提示应重视提高免疫系统的功能。同时，感染和治疗模型将有助于评估新的治疗方法，可以预防或逆转免疫抑制和感染并发症。
The spleen is one of the most important peripheral immune organs, which is frequently affected in infectious diseases. Infectious diseases can induce splenic alterations including splenic atrophy and functional alteration, while splenic atrophy may in turn interferes with recovery of infectious diseases. Angiostrongyliasis is an infectious disease by Angiostrongylus cantonensis (A. cantonensis), which invade non-permissive hosts, such as humans and mice, to cause severe damage to the central nervous system (CNS) and acute inflammatory response. A. cantonensis infection-induced CNS injury has been confirmed to be due to profound immunopathology derived from peripheral immune components. However, the mechanism of immunopathology remains largely unknown. Here, we found that A. cantonensis invaded non-permissive hosts such as mice in the brain, but not in the other peripheral organs. However, this infection induced severe spleen atrophy. We further recognized that this atrophy is associated with a decrease of total splenocyte number and disruption of splenic structure due to reduced proliferation and increased apoptotosis. These also resulted in deterioration of T cell profile in the periphery with a low CD4/CD8 ratio and B/T cell ratio, and increased ratio of CD4+CD25+Foxp3+Treg, CD8+CD28-T, and CD38+T lymphocyte of spleen. Albendazole treatment can alleviate spleen atrophy and set T cell immune reconstitution in some extend. Our data showed that A. cantonensis infection can cause splenic atrophy. These results are suggested to put more emphasis to improve the function of immune system. Meanwhile, infection and treatment model will be useful to evaluate new therapeutic approaches which can prevent or reverse immunosuppression and infectious complications.