Do catecholamines play a physiologic role in regulating corpus luteum function in the pseudopregnant rabbit?
Do catecholamines play a physiologic role in regulating corpus luteum function in the pseudopregnant rabbit?
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儿茶酚胺在调节假孕兔黄体功能中是否发挥生理作用？
DOI：
10.1095/biolreprod32.4.907
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发表时间：
1985
影响因子：
3.6
通讯作者：
Lucchesi,B
中科院分区：
文献类型：
--
作者：
Gadsby,JE;Keyes,PL;Schwartz,TS;Bill2nd,CH;Lucchesi,B
在这些研究中，13-肾上腺素能受体拮抗剂心得安用于体内雌激素反应的假孕兔，并测定血清孕酮浓度以监测黄体功能。实验1是为了确定心得安的有效剂量，1 mg/(kg‘h)sc心得安3h(血清中80 ng/ml)可产生足够水平的α-肾上腺素能受体阻滞剂，即对血压/异丙肾上腺素的量效关系有1000倍的抑制。在实验2中，在假孕7-8天、10-11天和13-14天“急性”给予心得安(P；1 mg/(kg‘h)sc)或生理盐水(对照，C)24小时，P或C动物的血清孕酮浓度在试验的任何一天都没有显著差异，尽管观察到每小时的波动。在实验3中，心得安的“慢性”治疗(为期4天)是通过sc(‘.血清中200-600 ng/ml)，在第13-17天。对照组动物只接受赋形剂颗粒。在整个治疗期间(第13-17天)以及第18天和第20天，P和C动物的血清孕酮浓度非常接近。我们得出结论：内源性儿茶酚胺在假孕兔黄体激素合成或黄体退化中不起主要作用。
In these studies the 13-adrenergic receptor antagonist propranolol was administered to estrogentreated hypophysectomized pseudopregnant rabbits in vivo, and serum progesterone concentratiOns were measured to monitor luteal function. In Experiment 1, which was designed to determine an effective dose of propranolol, 1 mg/(kg’h) sc propranolol for 3 h (‘\80 ng/ml in serum) gave an adequate level of a-adrenergic receptor blockade, ie, a 1000-fold inhibition of the blood pressure/isoproterenol dose-response relationship. In Experiment 2,“acute” administration of propranolol(P; 1 mg/(kg’h) sc) or saline(control, C) for 24 h on Days 7-8, 10-11, and 13-14 of pseudopregnancy did not produce any marked differences in serum progesterone concentrations in P or C animals on any of the days tested, although hourly fluctuations were observed. In Experiment 3,“chronic”(4-day) treatment with propranolol was achieved by the use of propranolol-containing pellets placed sc (‘.. 200-600 ng/ml in serum), on Days 13-17. Control animals received pellets of vehicle only. Serum progesterone concentrations were very similar in P and C animals throughout the period of treatment(Days 13-17) and on Days 18 and 20. We conclude that endogenous catecholamines play no major role in regulating luteal steroidogenesis or corpus luteum regression in the pseudopregnant rabbit.