Network-directed isolation of the cooperator Pseudomonas aeruginosa ZM03 enhanced the dibutyl phthalate degradation capacity of Arthrobacter nicotianae ZM05 under pH stress
Network-directed isolation of the cooperator Pseudomonas aeruginosa ZM03 enhanced the dibutyl phthalate degradation capacity of Arthrobacter nicotianae ZM05 under pH stress
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网络定向分离合作者铜绿假单胞菌ZM03增强了pH胁迫下烟草节杆菌ZM05的邻苯二甲酸二丁酯降解能力
DOI：
10.1016/j.jhazmat.2020.124667
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发表时间：
2021
影响因子：
13.6
通讯作者：
Lu Zhenmei
中科院分区：
文献类型：
--
作者：
Wang Xuejun;Wu Hao;Wang Xiaoyu;Wang Haixia;Zhao Kankan;Ma Bin;Lu Zhenmei
邻苯二甲酸二丁酯（DBP）是一种典型的土壤污染物，被广泛用作增塑剂。一种新的分离物，nicotianaeZM05，能有效降解DBP，但缺乏对恶劣环境的抗逆性。为了分离pH胁迫下菌株ZM05的有效合作者，探讨DBP对细菌群落结构和细菌间相互作用的影响，本研究通过提供外源DBP降解菌ZM05进行了微观实验。16S rRNA基因测序分析表明，DBP污染降低了微生物群落多样性，削弱了微生物之间的潜在相互作用，表现为共发生网络中的链接减少，平均程度降低，平均聚类系数降低。此外，子网络显示DBP改变了菌株ZM05与其他微生物之间的相互作用。在网络预测的基础上，分离出非降解细菌pseudomonasaeruginosazm03，并通过共培养实验证明，在pH胁迫下，菌株ZM05在DBP降解过程中具有正互作作用。菌株ZM03可以利用下游酸性代谢物减轻酸抑制，加速降解。该研究为细菌群落调节相互作用以适应DBP胁迫提供了确凿的证据，并为预测与降解细菌合作的微生物提供了新的见解。
Dibutyl phthalate (DBP), widely used as plasticizer, is a typical soil contaminant. A new isolate,Arthrobacter nicotianaeZM05, is efficient at degrading DBP but lacks stress resistance to adverse environments. In this study, to isolate effective cooperators of strain ZM05 under pH stress and explore the effects of DBP on the bacterial community structure and interaction between bacteria, a microcosm experiment was conducted by supplying the exogenous DBP-degrading bacteria ZM05. 16S rRNA gene sequencing analysis showed that DBP contamination decreased microbial community diversity and weakened potential interactions between microorganisms, evidenced by fewer links, lower average degree, and lower average clustering coefficients in the cooccurrence network. Furthermore, the subnetworks showed that DBP shifted the interactions between strain ZM05 and other microbes. Based on the prediction of the network, the nondegrading bacteriumPseudomonasaeruginosaZM03 was isolated and proven through coculture experiments to have a positive interaction with strain ZM05 during DBP degradation under pH stress. Strain ZM03 could utilize downstream acidic metabolites to alleviate acid inhibition and accelerate degradation. This study provides solid evidence that bacterial communities adjust their interactions to adapt to DBP stress and provides new insight into the prediction of microbes that are cooperative with degrading bacteria.