Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection
Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection
复制标题
DOI：
10.1016/j.tripleo.2006.05.010
复制
发表时间：
2007-02-01
期刊：
影响因子：
--
通讯作者：
Avila-Campos, Mario J.
中科院分区：
文献类型：
--
作者：
Tomazinho, Luiz Fernando;Avila-Campos, Mario J.
黑色厌氧杆菌，如普雷沃氏菌属。和卟啉单胞菌属。参与牙髓感染的病因和持续存在。本研究的目的是通过使用培养和聚合酶链反应（PCR）技术来评估这些物种在慢性牙髓感染中的患病率。通过消毒纸尖获取 100 名患有慢性牙髓感染的根管患者的样本。通过使用培养和PCR技术进行细菌鉴定。通过培养，在 33% 的样品中，P. intermedia-P.鉴定出黑质假单胞菌 (75.8%)、牙龈假单胞菌 (27.3%) 和牙髓假单胞菌 (9.1%)，通过 PCR，60% 的样品含有黑质假单胞菌 (43.3%)、牙龈假单胞菌 (43.3%)、中间假单胞菌 (31.7%) 和牙髓假单胞菌 (23.3%)。这些黑色厌氧杆单独或联合出现在慢性牙髓感染中似乎很常见。 PCR 是一种非常灵敏的技术，用于检测细菌细胞中的 DNA。培养只能揭示活细菌，对于识别少量细菌细胞不太敏感。
Black-pigmented anaerobic rods such as Prevotella spp. and Porphyromonas spp. are involved in the etiology and perpetuation of endodontic infections. The aim of this study was to evaluate the prevalence of these species in chronic enclodontic infections by using culture and polymerase chain reaction (PCR) techniques. Samples of 100 patients with root canals displaying chronic endodontic infections were obtained by sterilized paper points. Bacterial identification was performed by using culture and PCR techniques. By culture, in 33% of the samples, P. intermedia-P. nigrescens (75.8%), P. gingivalis (27.3%), and P. endodontalis (9.1 %) were identified, and by PCR 60% of the samples harbored P. nigrescens (43.3%), P. gingivalis (43.3%), P. intermedia (31.7%), and P. endodontalis (23.3%). The presence of these black-pigmented anaerobic rods alone or in association in chronic endodontic infections seems to be frequent. PCR is a very sensitive technique for detecting DNA from bacterial cells. Culturing is only able to reveal living bacteria and is less sensitive for the identification of low numbers of bacterial cells.