FISH Analysis of the W Chromosome in Bombyx mandarina and Several Other Species of Lepidoptera by Means of B. mori W-BAC Probes
FISH Analysis of the W Chromosome in Bombyx mandarina and Several Other Species of Lepidoptera by Means of B. mori W-BAC Probes
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DOI：
10.11416/jibs.76.1_1
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发表时间：
2007-02
影响因子：
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通讯作者：
A. Yoshido;Y. Yasukochi;F. Marec;H. Abe;K. Sahara
中科院分区：
文献类型：
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作者：
A. Yoshido;Y. Yasukochi;F. Marec;H. Abe;K. Sahara
利用6组不同的STS引物，从家蚕W染色体来源的细菌人工染色体文库中分离到7个W染色体来源的细菌人工染色体克隆，分别扩增出不同的雌性特异DNA片段。用19L6HW-BAC探针作为标准W染色体涂染器的双色荧光原位杂交(FISH)显示，7个新的W-BAC探针中有6个突出显示了家蚕的整个W染色体。除了W外，杂交信号还出现在常染色体的端粒和/或亚端粒区域。其余的W-BAC，20H9D，提供不连续的信号，出现在W染色体的末端和中央隔室。将代表的W染色体涂染BAC、19L6H和20H9D W-BAC杂交到野桑蚕雌体的染色体上。19L6HW-BAC与家蚕一样，结合在整个野桑叶的W染色体上。相反，野桑蚕W染色体的20H9D W-BAC信号与家蚕W染色体的20H9D W-BAC信号不同，表明在这两个亲缘关系密切的家蚕物种核型进化过程中W染色体发生了重排。进一步使用家蚕W-BAC探针对15种鳞翅目昆虫进行FISH分析，发现它们的性染色体和常染色体上没有杂交信号。我们的结果表明，家蚕W和其他鳞翅目物种的W染色体之间缺乏同源序列。
We newly isolated seven W chromosome derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by means of six different STS primer sets, which amplify each different female specific DNA fragment. Two color fluorescence in situ hybridization (FISH) with 19L6H W-BAC probe as a standard W-chromosome painter disclosed that six out of seven new W-BAC probes strongly highlighted the whole W chromosome in B. mori. Besides the W, hybridization signals occurred in telomeric and/or subtelomeric regions of the autosomes. The remaining W-BAC, 20H9D, provided discontinuous signals, which appeared in both terminal and central compartments of the W chromosome. A representative of W-chromosome painting BACs, 19L6H, and 20H9D W-BAC were hybridized to chromosome spreads of Bombyx mandarina females. The 19L6H W-BAC bound to the whole B. mandarina W chromosome as in B. mori. In contrast, 20H9D W-BAC signals in B. mandarina W chromosome were different from those in B. mori W, indicating rearrangements of the W chromosome during karyotype evolution of the two closely related Bombyx species. Further FISH analyses using B. mori W-BAC probes in 15 lepidopteran species showed no hybridization signals in their sex chromosomes as well as autosomes. Our results suggest the absence of homologous sequences between the Bombyx W and the W chromosomes of other lepidopteran species.