G-Quadruplex-Functionalized Gold Nanoparticles for a Real-Time Biomolecule Sensor with On-Demand Tunable Properties
G-Quadruplex-Functionalized Gold Nanoparticles for a Real-Time Biomolecule Sensor with On-Demand Tunable Properties
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DOI：
10.1021/acs.langmuir.2c00043
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发表时间：
2022-04-14
期刊：
影响因子：
3.9
通讯作者：
Maeda, Mizuo
中科院分区：
文献类型：
--
作者：
Chuaychob, Surachada;Fujita, Masahiro;Maeda, Mizuo
制备了G-四链体（G4）DNA功能化的金纳米粒子（AuNPs），用于生物分子凝血酶的新传感平台。凝血酶结合适体（TBA），它形成一个高度有序的G4结构，固定在金纳米粒子。通过凝血酶与G4 DNA的结合诱导颗粒聚集。因此，通过胶体系统从红色到紫蓝色的颜色变化来检测凝血酶。聚集不是由于颗粒之间通过凝血酶的桥接，而是由于G4 DNA的流动性和灵活性导致的空间排斥的减少。胶体稳定性变化快，峰位红移随凝血酶浓度变化而变化。该传感器对凝血酶靶标的特异性高于人血清中的主要蛋白质。检测灵敏度和分析性能成功地调整为按需传感器，线性度为10.0-40.0 nM。检测限和定量限分别为3.6和10.7 nM。
G-quadruplex (G4) DNA-functionalized gold nano-particles (AuNPs) were fabricated for a new sensing platform for a biomolecule, thrombin. Thrombin-binding aptamer (TBA), which forms a highly ordered G4 structure, was immobilized on AuNPs. The particles were induced to aggregate by binding of thrombin to G4 DNA. Thrombin was thus detected by the color change of the colloidal system from red to purple-blue. The aggregation was not due to the bridging between the particles through thrombin but to the reduction in steric repulsion attributable to the mobility and flexibility of G4 DNA. The change in the colloidal stability was quick and the bathochromic peak shift varied with the concentration of thrombin. The sensor showed a high specificity to the thrombin target over major proteins in human serum. The detection sensitivity and analytical performance were successfully tuned for an on-demand sensor with a linearity of 10.0-40.0 nM. The limits of detection and of quantification were 3.6 and 10.7 nM, respectively.