Corticotropin-releasing factor immunoreactivity increases in the cerebellar climbing fibers in the novel ataxic mutant mouse, Pogo
Corticotropin-releasing factor immunoreactivity increases in the cerebellar climbing fibers in the novel ataxic mutant mouse, Pogo
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DOI：
10.1111/j.1439-0264.2005.00646.x
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发表时间：
2006-04-01
期刊：
影响因子：
--
通讯作者：
Fukui, Y
中科院分区：
文献类型：
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作者：
Jeong, YG;Chung, SH;Fukui, Y
共济失调pogo小鼠（pogo/pogo）是一种新的神经系统突变型小鼠，它是从韩国野生小鼠中分离出来的近交系（KJR/MsKist）。病理表现包括难以保持正常姿势、肢体间协调失败和不能直立行走。在这项研究中，我们使用免疫组织化学方法检查了pogo突变小鼠小脑中促肾上腺皮质激素释放因子（CRF）免疫反应性小脑攀爬纤维的分布及其向酪氨酸羟化酶（TH）免疫反应性浦肯野细胞的投射。在pogo/pogo小鼠中，爬行纤维的一个子集比对照组对CRF的染色更强烈。此外，共济失调pogo小鼠，下橄榄核投射攀爬纤维的神经元也更强烈的CRF染色比对照组。在pogo/pogo小鼠，TH免疫反应位于浦肯野细胞，而没有TH的表达被发现在控制。CRF和TH的双重免疫染色在pogo/pogo小脑显示，TH-免疫反应浦肯野细胞的分布对应于CRF-免疫反应攀爬纤维的终端领域，但不是CRF-免疫反应苔藓纤维。因此，我们认为，CRF水平的增加可能会改变目标浦肯野细胞的功能，它是相关的共济失调表型在pogo突变小鼠。
The ataxic pogo mouse (pogo/pogo) is a novel neurological mutant, which was derived as an inbred strain (KJR/MsKist) from a Korean wild mouse. The pathological manifestations include a difficulty in maintaining a normal posture, the failure of inter-limb coordination and an inability to walk straight. In this study, we examined the distribution of corticotropin-releasing factor (CRF) immunoreactive cerebellar climbing fibres and their projections to tyrosine hydroxylase (TH) immunoreactive Purkinje cells in the cerebellum of the pogo mutant mouse using immunohistochemistry. In the pogo/pogo mouse, a subset of climbing fibres was stained more intensely for CRF than in the control. Moreover, ataxic pogo mouse, neurons of the inferior olivary nucleus projecting climbing fibres were also more intensely stained for CRF than in the control. In the pogo/pogo mouse, TH immunoreactivity was located in the Purkinje cells, whereas no TH expression was found in the control. Double immunostaining for CRF and TH in the pogo/pogo cerebellum revealed that the distribution of TH-immunoreactive Purkinje cells corresponded to terminal fields of CRF-immunoreactive climbing fibres but not to the CRF-immunoreactive mossy fibres. Therefore, we suggest that an increase of CRF level may alter the function of targeted Purkinje cells and that it is related to the ataxic phenotype in the pogo mutant mouse.