Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples
Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples
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DOI：
10.1089/fpd.2009.0324
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Lobato, Zelia I. P.
中科院分区：
文献类型：
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作者：
Abrahao, Jonatas S.;Oliveira, Tercia M. L.;Lobato, Zelia I. P.
痘苗病毒(VACV)会在奶牛和人类身上引起红斑性皮损，它与巴西的几次牛痘疫情有关。目前，没有关于受VACV影响地区生产的牛奶安全性的数据。在这项研究中，在牛痘暴发期间采集了47个牛奶样本，并将其提交给病毒分离、DNA检测和保守的tk基因的核苷酸测序。在鸡蛋的绒毛尿囊膜上出现典型的白斑，与鸡胚成纤维细胞中病毒的细胞病变效应和系统发育数据相关联，强烈表明牛奶受到VACV的污染。这是从牛奶中检出和分离出VACV的第一份报告。
The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.