Effect of Trichoderma asperellum strain T34 and glucose addition on iron nutrition in cucumber grown on calcareous soils
Effect of Trichoderma asperellum strain T34 and glucose addition on iron nutrition in cucumber grown on calcareous soils
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DOI：
10.1016/j.soilbio.2012.06.020
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发表时间：
2013-02-01
影响因子：
9.7
通讯作者：
Delgado, Antonio
中科院分区：
文献类型：
--
作者：
de Santiago, Ana;Garcia-Lopez, Ana M.;Delgado, Antonio
真菌棘孢木霉T34是一种生物防治剂，已被证明可以提高植物对铁的吸收。本研究的目的是研究T34对土壤中黄瓜植株铁有效性的贡献，以及这种潜在的供应如何受到土壤性质，特别是土壤微生物活性的影响。为此，进行了两次完全随机实验，涉及三个因素：土壤（LB 9、LB 11和LB 14）、接种T34的植物（接种和未接种T34）和葡萄糖供应，以实现引发效应（供应和不供应）。接种T34能有效地促进植株生长和地上部Fe、Cu的累积。这种增加是不相关的增加积累或浓度的Fe或Cu在根中。葡萄糖的加入只有效地增加铁积累在地上部分的植物生长在土壤中的有机碳含量和天然β-葡萄糖苷酶活性最低（LB 9土壤）。T34对植物地上部分铁浓度的影响因土壤和葡萄糖添加而异。在LB 9土壤中，同时施用T34和葡萄糖增加了真菌的发育，并导致植物枝条中的铁浓度增加，与不施用T34和葡萄糖的对照相比。在土壤中，β-葡萄糖苷酶活性增加葡萄糖添加（LB 14土壤），这种除了导致增加的铁浓度在地上部分的植物，从而揭示了增加土壤微生物活性的影响，提高铁吸收植物; T34单独产生类似的效果，在这种土壤中与葡萄糖实现的铁浓度。葡萄糖或T34没有影响铁浓度在地上部分的植物生长在土壤中的最高的铁有效性指数（LB 11），从而表明T34或微生物活性的效果可以不太明显时，养分的可用性不受限制。(C)2012爱思唯尔有限公司保留所有权利。
The fungus Trichoderma asperellum T34 is a biological control agent which has been shown to enhance Fe uptake by plants. The objective of this research was to study the contribution of T34 to Fe availability to cucumber plants in soils and how this potential supply can be affected by soil properties and in particular, by soil microbial activity. To this end, a completely randomised experiment was performed twice involving three factors: soil (LB9, LB11, and LB14), plant inoculation with T34 (inoculated and non-inoculated with T34), and glucose supply to achieve a priming effect (supply and no supply). Inoculation with T34 was effective in increasing plant growth and total accumulation of Fe and Cu in aerial parts. This increase was not related to an increased accumulation or concentration of Fe or Cu in roots. Glucose addition was only effective in increasing Fe accumulation in the aerial parts of plants grown in the soil with the lowest organic C content and native beta-glucosidase activity (LB9 soil). The effect of T34 on Fe concentration in the aerial parts of plants differed depending on the soil and the glucose addition. In the LB9 soil, the simultaneous application of T34 and glucose increased the development of the fungus and resulted in an increased Fe concentration in plant shoots when compared to the control without T34 and glucose. In the soil where beta-glucosidase activity was increased by glucose addition (LB14 soil), this addition resulted in an increased Fe concentration in aerial parts of plants, thus revealing the effect of increased soil microbial activity on improving Fe uptake by plants; T34 alone produced a similar effect on Fe concentration to that achieved with glucose in this soil. Glucose or T34 did not affect Fe concentration in aerial parts of plants grown in the soil with the highest Fe availability index (LB11), thus showing that the effect of T34 or microbial activity can be less evident when the availability of nutrients is not so restricted. (C) 2012 Elsevier Ltd. All rights reserved.