Measurements of the equivalent whole-body dose during radiation therapy by cytogenetic methods
Measurements of the equivalent whole-body dose during radiation therapy by cytogenetic methods
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DOI：
10.1088/0031-9155/44/5/314
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发表时间：
1999-05-01
影响因子：
3.5
通讯作者：
Tsujii, H
中科院分区：
文献类型：
--
作者：
Durante, M;Yamada, S;Tsujii, H
可以使用不同的生物物理模型来估计局部接触后的等效全身剂量。计算应与生物剂量学数据进行比较，但测量是复杂的有丝分裂选择后，在靶细胞局部照射诱导。本文应用间期染色体涂染技术，测量了放射治疗中外周血淋巴细胞染色体畸变，并估算了全身吸收的等效剂量。通过在calyculin A中孵育，在刺激的淋巴细胞中诱导过早的染色体凝聚，并将载玻片与人类染色体2和4特异性的全染色体DNA探针原位杂交。根据个体给药前体外校准曲线，使用相互交换估计等效全身剂量。等效全身剂量随分次数的增加而增加，并在高分次数时达到平台。染色体畸变率取决于字段大小，肿瘤的位置和同期化疗。结果表明，间期染色体绘画是一种简单的技术，能够提供一个可靠的估计，在治疗性局部照射的等效全身吸收剂量。
Estimates of equivalent whole-body dose following partial body exposure can be performed using different biophysical models. Calculations should be compared with biodosimetry data, but measurements are complicated by mitotic selection induced in target cells after localized irradiation. In this paper we measured chromosomal aberrations in peripheral blood lymphocytes during radiotherapy, and estimated the equivalent whole-body dose absorbed, by using the novel technique of interphase chromosome painting. Premature chromosome condensation was induced in stimulated lymphocytes by incubation in calyculin A, and slides were hybridized in situ with whole-chromosome DNA probes specific for human chromosomes 2 and 4. Reciprocal exchanges were used to estimate the equivalent whole-body dose, based on individual pre-treatment in vitro calibration curves. Equivalent whole-body dose increased as a function of the number of fractions, and reached a plateau at high fraction numbers. Chromosomal aberration yields were dependent on field size, tumour position and concurrent chemotherapy. Results suggest that interphase chromosome painting is a simple technique able to give a reliable estimate of the equivalent whole-body dose absorbed during therapeutic partial-body irradiation.