Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR.
Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR.
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CtxA-霍乱弧菌Texas Star-SR菌株霍乱毒素基因的分子克隆和核苷酸序列分析。
DOI：
10.1128/iai.58.12.4142-4144.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
McIntosh,MA
中科院分区：
文献类型：
--
作者：
Brickman,TJ;Boesman-Finkelstein,M;Finkelstein,RA;McIntosh,MA
克隆了霍乱弧菌El Tor 3083菌株及其CtxA衍生物Texas Star-SR的ctx操纵子，并比较了它们的核苷酸序列。鉴定了Texas Star-SR ctxA顺反子中的单个错义突变，其导致258个氨基酸的CtxA前体的位置191处的丙氨酸被苏氨酸取代。免疫印迹分析检测到的突变CtxA细胞内早期的文化周期，但在任何生长阶段的细胞外。
The ctx operons from the Vibrio cholerae El Tor strain 3083 and its CtxA- derivative Texas Star-SR were cloned, and their nucleotide sequences were compared. A single missense mutation in the Texas Star-SR ctxA cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid CtxA precursor was identified. Immunoblot analysis detected the mutant CtxA intracellularly early in the culture cycle but not extracellularly at any growth stage.