Effect of pluronic-block copolymers on the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine-DNA complexes
Effect of pluronic-block copolymers on the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine-DNA complexes
复制标题
DOI：
10.1042/ba20020123
复制
发表时间：
2003-06-01
影响因子：
2.8
通讯作者：
Kuo, JHS
中科院分区：
文献类型：
--
作者：
Kuo, JHS
非病毒载体的血清稳定性是成功体内基因递送的关键因素。测试了由亲水性环氧乙烷和疏水性环氧丙烷嵌段组成的Pluronic嵌段共聚物，以防止NIH/3 T3细胞中血清介导的聚乙烯亚胺（PEI）-DNA复合物的基因转移抑制的减少。在本研究中使用的六种不同类型的普朗尼克的亲水亲油平衡（HLB）的顺序为F68 > F127> P105 > P94 > L122 > L 61。NIH/3 T3细胞的转染活性与PEI-DNA复合物含有Pluronics具有较高的HLB值显示出显着的改善，在血清培养基中的基因表达水平从10至50%胎牛血清相比，单独的PEI-DNA复合物。此外，PEI/DNA分散体中具有较高HLB的较高浓度（1%和3%）的Pluronics在抵抗血清组分方面提供了比在较低浓度（0.1%）下获得的那些更强的空间位阻。这些结果表明，非病毒载体与较高的HLB值的普朗尼克类药物可用作潜在的载体，在体内传递的DNA。
Serum stability of non-viral vectors is a crucial factor for successful in vivo gene delivery. Pluronic-block copolymers consisting of hydrophilic ethylene oxide and hydrophobic propylene oxide blocks were tested to prevent the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine (PEI)-DNA complexes in NIH/3T3 cells. The order of hydrophilic-lipophilic balance (HLB) of six different types of Pluronics used in this study was F68 > F 127 > P105 > P94 > L122 > L61. Transfection activities of NIH/3T3 cells with PEI-DNA complexes containing Pluronics with higher HLB showed marked improvement of gene-expression levels in serum media from 10 to 50% fetal bovine serum compared with PEI-DNA complexes alone. Also, higher concentrations (1 and 3%) of Pluronics with higher HLB in the PEI/DNA dispersion provided a stronger steric hindrance in resisting serum components than those obtained in a lower concentration (0.1%). These results suggested that non-viral vectors incorporated with higher HLB of Pluronics may be used as potential vehicles for in vivo delivery of DNA.